Fluorescent quantitative PCR detection kit and detection method for shrimp infectious myonecrosis virus

A detection kit and a technique for muscle necrosis, which are applied in the directions of microorganism-based methods, microorganism determination/inspection, biochemical equipment and methods, etc., can solve the problem of low sensitivity, and achieve sensitive methods, high popularization and application value, and easy operation. Effect

Inactive Publication Date: 2015-12-16
LUDONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the sensitivity of existing fluorescent quantitative PCR detection method is not high, provide the fluorescent quantitative PCR detection kit of prawn infectious myonecrosis virus, also provide the detection method that utilizes this kit to detect simultaneously, The detection method is sensitive, specific and easy to operate

Method used

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  • Fluorescent quantitative PCR detection kit and detection method for shrimp infectious myonecrosis virus
  • Fluorescent quantitative PCR detection kit and detection method for shrimp infectious myonecrosis virus
  • Fluorescent quantitative PCR detection kit and detection method for shrimp infectious myonecrosis virus

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Embodiment 1

[0027] The fluorescent quantitative PCR detection kit for shrimp infectious myonecrosis virus comprises a box body 1, and a tube hole support 5 is arranged in the box body 1, and the following pipe fittings are arranged on the tube hole support 5:

[0028] (1) Fluorescent quantitative PCR reaction solution tube 6, which contains 2× fluorescent quantitative PCR buffer, a mixture of positive and negative primers for shrimp infectious myonecrosis virus and fluorescently labeled TaqMan probes;

[0029] Forward primer sequence: 5'-GATGGCAGCACGTCAAAATG-3';

[0030] Reverse primer sequence: 5'-CAGCTGTTCCTGGGACTTCCT-3';

[0031] TaqMan probe sequence: 5'-CTATTTCCTCCACTAATTCAGAGC-3', the 5' end of the probe sequence is labeled with FAM, and the 3' end is labeled with TAMRA;

[0032] (2), deionized water pipe 3, filled with sterile deionized water;

[0033] (3) Negative control tube 4, containing SPF (specific pathogen free) prawn nucleic acid;

[0034] (4), standard quality control ...

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Abstract

The invention relates to a detection kit and a detection method for shrimp infectious myonecrosis virus, belonging to the technical field of detection of marine biological pathogens. Including the box body, the tube hole bracket is arranged in the box body, and the following tubes are placed on the tube hole bracket: (1), fluorescent quantitative PCR reaction solution tube; forward primer sequence; reverse primer sequence; TaqMan probe sequence; (2) ), deionized water tubes, containing sterile deionized water; (3), negative control tubes, containing SPF (specific pathogen free) prawn nucleic acid; (4), standard quality tubes, containing infectious myonecrosis virus 107, 106, 105, 104, 103, 102, 10, 1 copy cDNA positive clone. The invention has high popularization and application value, and the detection method is sensitive, specific and easy to operate.

Description

technical field [0001] The invention relates to a detection kit and a detection method for shrimp infectious myonecrosis virus, belonging to the technical field of detection of marine biological pathogens. Background technique [0002] Infectious myonecrosis is a new viral infectious disease that endangers the production of prawns discovered in Brazil in 2002. In 2004, Dr. LightnerDV of the University of Arizona found that the disease was caused by a new virus and named it Infectious myonecrosis virus (IMNV for short). The novel virus is a double-stranded RNA virus belonging to the family Holoviridae. The susceptible shrimp species of shrimp infectious myonecrosis virus is Penaeus vannamei, mainly juvenile shrimp infected 60-80 days old. The virus can cause muscle tissue necrosis in the whole body of infected prawns. Under normal circumstances, pathological death occurs slowly and the mortality rate is not high, but there are deaths throughout the breeding process, and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12R1/93
Inventor 闫冬春刘鸿玲
Owner LUDONG UNIVERSITY
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