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Method for extracting catalase from yak liver

A catalase and extraction method technology, applied in the field of enzyme products, can solve the problems of increased purification difficulty, long production cycle, unstable enzyme activity, etc., to reduce the use of chemical reagents, improve the safety of use, and reduce production costs Effect

Inactive Publication Date: 2012-07-11
兰州天禾生物催化技术有限公司
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Problems solved by technology

Liver extraction of CAT has been industrialized in China, but the addition of organic solvents and inorganic salts in the traditional extraction process increases the difficulty of subsequent enzyme purification, the product purity is not high, the enzyme activity is unstable, the yield is low, and the production cycle is long. In addition, the traditional drying technology The enzyme protein is seriously damaged, and the products produced are not food-grade catalase

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  • Method for extracting catalase from yak liver

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Embodiment 1

[0018] Example 1: A kind of method taking fresh yak liver as raw material to extract catalase, its implementation steps are as follows:

[0019] (1) Selection of raw materials: Fresh yak livers collected from slaughterhouses are selected as raw materials.

[0020] (2) Pretreatment of raw materials: fresh yak liver was artificially removed with white tissue, ground repeatedly 3 times with a meat grinder, and then ground into liver pulp with a colloid mill with a gap of 0.9-1mm for later use.

[0021] (3) Extraction of catalase: mix the prepared liver pulp with deionized water at a ratio of 1:6, adjust the pH to 6.3-6.5, place the mixed liquid in a constant temperature vacuum extraction tank, and extract at a constant temperature The temperature is 30°C, the pressure inside the tank is 0.08-0.09 MPa, and the extraction time is 60 minutes.

[0022] (4) Separation of catalase: Use a disc centrifuge to separate the impurities in the extract. The speed of the centrifuge is 5000 r / ...

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Abstract

The invention discloses a method for extracting catalase from yak liver, and belongs to the technical field of enzyme products. The market price of the catalase is higher, and the catalase does not belong to food-grade catalase. The method comprises the steps of: crushing the yak liver by adopting a colloid mill, extracting the catalase by using a thermostatic vacuum extraction tank, separating the catalase by a disc centrifuge, purifying the catalase with a membrane separation technology, and then drying the catalase by a spray freeze drying machine, thereby preparing food-grade and high-purity catalase, wherein the enzyme activity of the food-grade and high-purity catalase can reach 50000U / mg. According to the method, the consumption of chemical reagents is reduced in the extraction process, and the use safety of the product is improved, and the demand of food industry on the catalase can be met.

Description

technical field [0001] The invention relates to a catalase method, belongs to the technical field of enzyme products, and relates to a method for extracting catalase from yak liver. Background technique [0002] Catalase (Hydrogen Peroxidase), also known as Catalase (CAT), has a molecular weight of 225,000 to 250,000 Daltons. It is an oxidoreductase widely present in animals, plants, and microorganisms. It is specific for hydrogen peroxide. The substrate is catalyzed and decomposed into water and oxygen, which is an important biological enzyme preparation product. At present, catalase, as a biological enzyme product, is widely used in food, medicine, textile, papermaking, environmental protection and other fields. With the increasingly serious problem of food safety in our country, hydrogen peroxide, as a non-toxic and harmless disinfectant, is used more and more for food sterilization in my country, and catalase is also accompanied by the use of hydrogen peroxide. The mark...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08
Inventor 郭天力高维东李晓鹏贺晓玲马新华马林安志刚
Owner 兰州天禾生物催化技术有限公司
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