Quality control method of Chinese medicinal hindu datura flower capsule for treating psoriasis
A quality control method, the technology of Yangjinhua, which is applied in measurement devices, color/spectral characteristic measurement, instruments, etc., can solve problems such as inability to cure and limited curative effect.
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Embodiment 1
[0034] A quality control method of Yangjinhua Capsules, a traditional Chinese medicine for the treatment of psoriasis. The method is divided into eight steps: The first step is to identify 3-O-β-D in the effective parts of Yangjinhua Capsules by thin layer chromatography. -The method of grape pyranosyl 1 grape pyranosyl-7-O-α-L and 2 rhamnopyranosyl kaempferol, the second step is to identify the effective part of the foreign traditional Chinese medicine Jinhua capsule Zhongyang Aureoside C is 5α, 12β, 27-trihydroxy-6α, 7α-epoxy is 20R, 22R, -1- keto- drunk-2, 24-dienolactone-27-O-β-D- In the method of glucopyranoside, the third step is to identify the alkaloids in the effective part of the traditional Chinese medicine Yangjinhua capsule by thin-layer chromatography, and the fourth step is to determine the total flavonoids in the effective part of the traditional Chinese medicine Yangjinhua capsule by ultraviolet spectrophotometry. The fifth step is the ultraviolet spectrophotom...
Embodiment 2
[0036] The quality control method of the traditional Chinese medicine Yangjinhua capsule for the treatment of psoriasis described in embodiment 1, said thin-layer chromatography for identifying 3-O-β-D-glucopyranosyl 1 in the effective part of the traditional Chinese medicine Yangjinhua capsule The method of grape pyranosyl-7-O-α-L and 2 rhamnopyranosyl kaempferol,
[0037] First take 1 capsule of Chinese medicine Yangjinhua capsule, add 2-3ml of methanol, close it, and extract with ultrasound for 30 minutes, let stand, filter, and dilute the filtrate to 4-6ml as the test solution, then take another reference 3- O-β-D-glucopyranosyl 2mg, namely 1 glucopyranosyl-7-O-α-L, 2 rhamnopyranosyl kaempferol, add methanol and dilute to a 9-11ml volumetric flask , Make the reference solution; according to the thin-layer chromatography test, draw the test solution and the reference solution, respectively point on the same polyamide film plate, using methanol-water-glacial acetic acid 4:6:0.1...
Embodiment 3
[0039] The quality control method of the traditional Chinese medicine Yangjinhua Capsules for the treatment of psoriasis described in Example 1, wherein the thin-layer chromatography is used to identify the effective parts of the traditional Chinese medicine Yangjinhua Capsules, namely 5α, 12β, 27-trihydroxyl -6α, 7α-epoxy, ie 20R, 22R,-1-keto-solanum-2, 24-dienolactone-27-O-β-D-glucopyranoside,
[0040] Take 20 capsules of traditional Chinese medicine Yangjinhua capsules, add 90-110ml of methanol, plug tightly, ultrasonically extract for 25-35 minutes, let stand, filter, make the recovered filtrate, transfer out with 2-10ml to make water transfer liquid; water The transferred solution is passed through D941 macroporous weakly basic acrylic anion exchange resin, and the water eluate is collected and dried, and transferred to a constant volume with methanol in a 9-11ml volumetric flask as the test solution; take another reference substance, damask flower Glycoside C5α,12β,27-trihy...
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