Riboflavin sodium phosphate compound

Abstract

The invention relates to a riboflavin sodium phosphate compound, which exists in a crystal form. X-ray powder diffraction measured by Cu-K alpha rays shows that characteristic peaks exist at 2Theta of 12.4 degrees, 13.3 degrees, 14.7 degrees, 16.1 degrees, 16.9 degrees, 18.1 degrees, 18.9 degrees, 19.9 degrees, 20.9 degrees, 21.5 degrees, 22.7 degrees, 24.0 degrees, 24.7 degrees, 25.4 degrees, 26.7 degrees, 27.4 degrees and 28.2 degrees. The riboflavin sodium phosphate compound has main particle sizes of 5 to 15 mu m, preferably 6 to 12 mu m, and the distribution width of 2 to 20 mu m, preferably 3 to 18 mu m. A stability experiment shows that the riboflavin sodium phosphate crystal compound has high stability and better water solubility.

Description

technical field

[0001] The invention relates to a riboflavin phosphate, in particular to a riboflavin sodium phosphate crystal compound. Background technique

[0002] Riboflavin (riboflavin), also known as VB2, chemically named 6,7-dimethyl-10-(1′-D-ribitolyl)-isoalloxazine, is an important part of the enzyme system of human metabolism , which is an important prosthetic group of many enzyme systems in the body - the constituents of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN), participating in the energy metabolism and material metabolism of the body . If the body is severely deficient in riboflavin, it will affect the normal oxidation in the body, cause material metabolism disorders, and cause a series of diseases in the body, such as: angular cheilitis, glossitis, seborrheic dermatitis of the nose and face; eyes Corneal redness, hyperemia, etc. Riboflavin sodium phosphate (riboflavin sodium phosphate, riboflavin5′-phosphate sodium) is an important d...

Examples

Embodiment 1

[0033] First, add riboflavin sodium phosphate solid into 1000ml of distilled water at a mass ratio of 1:100, dissolve evenly, adjust the pH value of the solution to 9.5, and cool the solution to 7°C;

[0034]Then, under the sound field that the frequency is 30KHz and the output power is 40W, add the mixed solution of dehydrated alcohol, dehydrated acetone and anhydrous 1,2-propanediol identical to the riboflavin solid solution temperature, and stir while adding; The volume ratio of water ethanol, anhydrous acetone and anhydrous 1,2-propanediol is 1:0.5:0.05. The speed that the mixed solvent is added is v=M / 500, wherein M=1000ml, v=2 milliliters / minute, add completely in 3 hours. After adding the mixed solvent, the sound field was removed, and the crystal was grown at 20° C. for 1 hour.

[0035] Finally, filter, wash the filter cake with ethanol, and vacuum-dry for 4 hours to obtain riboflavin sodium phosphate crystals.

[0036] The X-ray powder diffraction measured by using ...

Embodiment 2

[0038] First, add riboflavin sodium phosphate solid into 6000ml of distilled water at a mass ratio of 1:750, dissolve evenly, adjust the pH value of the solution to 9.5, and cool the solution to 5°C;

[0039] Then, under the sound field that the frequency is 30KHz and the output power is 40W, add the mixed solution of dehydrated alcohol, dehydrated acetone and anhydrous 1,2-propanediol identical to the riboflavin solid solution temperature, and stir while adding; The volume ratio of water ethanol, anhydrous acetone and anhydrous 1,2-propanediol is 1:0.4:0.075. The speed of adding the mixed solvent is v=M / 600, wherein M=6000 milliliters, the speed v=10 milliliters / minute, and add completely in 3 hours. After adding the mixed solvent, the sound field was removed, and the crystal was grown at 22° C. for 4 hours.

[0040] Finally, filter, wash the filter cake with ethanol, and vacuum-dry for 3-6 hours to obtain riboflavin sodium phosphate crystals.

[0041] X-ray powder diffract...

Embodiment 3

[0043] First, add riboflavin sodium phosphate solid into 3000ml of distilled water at a mass ratio of 1:50, dissolve evenly, adjust the pH value of the solution to 9.5, and cool the solution to 10°C;

[0044] Then, under the sound field that the frequency is 30KHz and the output power is 40W, add the mixed solution of dehydrated alcohol, dehydrated acetone and anhydrous 1,2-propanediol identical to the riboflavin solid solution temperature, and stir while adding; The volume ratio of water ethanol, anhydrous acetone and anhydrous 1,2-propanediol is 1:0.4:0.1. The speed of adding the mixed solvent is v=M / 600, wherein M=3000 ml, the speed v=5 ml / min, and the addition is completed in 4 hours. Remove the sound field after adding the mixed solvent, and let the crystal grow at 25°C for 1 to 5 hours.

[0045] Finally, filter, wash the filter cake with ethanol, and vacuum-dry for 4 hours to obtain riboflavin sodium phosphate crystals.

[0046] X-ray powder diffraction measured by usi...

technical field

[0001] The invention relates to a riboflavin phosphate, in particular to a riboflavin sodium phosphate crystal compound. Background technique

[0002] Riboflavin (riboflavin), also known as VB2, chemically named 6,7-dimethyl-10-(1′-D-ribitolyl)-isoalloxazine, is an important part of the enzyme system of human metabolism , which is an important prosthetic group of many enzyme systems in the body - the constituents of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN), participating in the energy metabolism and material metabolism of the body . If the body is severely deficient in riboflavin, it will affect the normal oxidation in the body, cause material metabolism disorders, and cause a series of diseases in the body, such as: angular cheilitis, glossitis, seborrheic dermatitis of the nose and face; eyes Corneal redness, hyperemia, etc. Riboflavin sodium phosphate (riboflavin sodium phosphate, riboflavin5′-phosphate sodium) is an important d...

Examples

Embodiment 1

[0033] First, add riboflavin sodium phosphate solid into 1000ml of distilled water at a mass ratio of 1:100, dissolve evenly, adjust the pH value of the solution to 9.5, and cool the solution to 7°C;

[0034]Then, under the sound field that the frequency is 30KHz and the output power is 40W, add the mixed solution of dehydrated alcohol, dehydrated acetone and anhydrous 1,2-propanediol identical to the riboflavin solid solution temperature, and stir while adding; The volume ratio of water ethanol, anhydrous acetone and anhydrous 1,2-propanediol is 1:0.5:0.05. The speed that the mixed solvent is added is v=M / 500, wherein M=1000ml, v=2 milliliters / minute, add completely in 3 hours. After adding the mixed solvent, the sound field was removed, and the crystal was grown at 20° C. for 1 hour.

[0035] Finally, filter, wash the filter cake with ethanol, and vacuum-dry for 4 hours to obtain riboflavin sodium phosphate crystals.

[0036] The X-ray powder diffraction measured by using ...

Embodiment 2

[0038] First, add riboflavin sodium phosphate solid into 6000ml of distilled water at a mass ratio of 1:750, dissolve evenly, adjust the pH value of the solution to 9.5, and cool the solution to 5°C;

[0039] Then, under the sound field that the frequency is 30KHz and the output power is 40W, add the mixed solution of dehydrated alcohol, dehydrated acetone and anhydrous 1,2-propanediol identical to the riboflavin solid solution temperature, and stir while adding; The volume ratio of water ethanol, anhydrous acetone and anhydrous 1,2-propanediol is 1:0.4:0.075. The speed of adding the mixed solvent is v=M / 600, wherein M=6000 milliliters, the speed v=10 milliliters / minute, and add completely in 3 hours. After adding the mixed solvent, the sound field was removed, and the crystal was grown at 22° C. for 4 hours.

[0040] Finally, filter, wash the filter cake with ethanol, and vacuum-dry for 3-6 hours to obtain riboflavin sodium phosphate crystals.

[0041] X-ray powder diffract...

Embodiment 3

[0043] First, add riboflavin sodium phosphate solid into 3000ml of distilled water at a mass ratio of 1:50, dissolve evenly, adjust the pH value of the solution to 9.5, and cool the solution to 10°C;

[0044] Then, under the sound field that the frequency is 30KHz and the output power is 40W, add the mixed solution of dehydrated alcohol, dehydrated acetone and anhydrous 1,2-propanediol identical to the riboflavin solid solution temperature, and stir while adding; The volume ratio of water ethanol, anhydrous acetone and anhydrous 1,2-propanediol is 1:0.4:0.1. The speed of adding the mixed solvent is v=M / 600, wherein M=3000 ml, the speed v=5 ml / min, and the addition is completed in 4 hours. Remove the sound field after adding the mixed solvent, and let the crystal grow at 25°C for 1 to 5 hours.

[0045] Finally, filter, wash the filter cake with ethanol, and vacuum-dry for 4 hours to obtain riboflavin sodium phosphate crystals.

[0046] X-ray powder diffraction measured by usi...

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