Method for extracting oil tea cake proteins
A technology of camellia oil cake and extraction method, which is applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc., can solve problems such as low extraction rate and achieve the effect of improving utilization rate
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[0041] According to a preferred embodiment of the method of the present invention, in order to further improve the yield of the camellia cake meal protein, the preparation method further comprises: using part or all of the camellia cake meal in step (1) with the solids obtained in step (2) Replace and repeat steps (1) to (4); and / or
[0042] Replace all the camellia cake in step (1) with the solids obtained in step (2) and repeat the operations of steps (1) to (4) 2-3 times.
[0043] According to a preferred embodiment of the method of the present invention, in order to further increase the yield of the camellia cake meal protein, the method further comprises: stirring the first mixture for 5-50 minutes before irradiating the first mixture with ultrasonic waves, more preferably 10-30 minutes.
[0044] According to a preferred embodiment of the method of the present invention, in order to further improve the quality of the oil-tea cake meal protein, the method further includes: freez...
Embodiment 1
[0052] This example is used to illustrate the extraction method of camellia oleifera cake protein provided by the present invention.
[0053] (1) Camellia cake with a particle size of 100-120 mesh (purchased from the Institute of Subtropical Forestry, Chinese Academy of Forestry, with an oil content of 8% by weight, without removing tea saponin, according to the literature (Wu Xiaochun, Journal of Yichun University, 2008 The protein content was determined to be 16% by weight according to the method in the fourth issue of 2008, 29-30).) 100 grams were mixed with 33 grams of absolute ethanol and 2700 grams of distilled water and sodium hydroxide was added to adjust the pH to 9.8 to obtain the first mixture. And the first mixture was stirred at room temperature for 20 minutes. Under the conditions of power 950W (relative to the entire first mixture), frequency of 40 kHz and temperature of 40° C., the stirred first mixture was irradiated with ultrasonic waves for 55 minutes to obtain...
Embodiment 2
[0062] This example is used to illustrate the method of the present invention. In this example, the protein of camellia cake meal is extracted according to the following steps:
[0063] (1) Camellia cake with a particle size of 100-120 mesh (purchased from the Institute of Subtropical Forestry, Chinese Academy of Forestry, with an oil content of 8% by weight, according to the literature (Wu Xiaochun, Journal of Yichun University, 2008, Issue 4, 29- 30 pages) method to determine its protein content is 16% by weight) 100 grams, 20 grams of absolute ethanol and 2500 grams of distilled water are mixed and sodium hydroxide is added to adjust the pH to 9.5 to obtain the first mixture, and the first mixture is kept at room temperature Stir for 15 minutes. Under the conditions of a power of 850W (relative to the entire first mixture), a frequency of 25 kHz and a temperature of 35° C., the stirred first mixture was irradiated with ultrasonic waves for 30 minutes to obtain a product after ...
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Abstract
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