Correction method of deoxyribonucleic acid high-pass sequencing data for gene expression detection

Abstract

The invention belongs to the field of the detection of molecular biological information, in particular to a correction method for improving the accuracy of deoxyribonucleic acid (DNA) high-pass sequencing gene expression detection data. The correction method comprises the steps as follows: (1) gene expression DNA sequencing detection data are collected, and a gene expression DNA high-pass sequencing detection data correction model is established; (2) gene expression values measured by gene chips are collected; (3) model parameters in the gene expression high-pass sequencing correction model are determined by adopting correlation analysis; and (4) corrected gene expression values are generated by the gene expression DNA high-pass sequencing detection data correction model of which the model parameters are determined. The correction method has the advantages that sequence alignment mapping errors caused when DNA sequencing values are corrected by utilizing the correction model are estimated and compensated, so that the detection errors are reduced, and the detection efficiency is effectively improved on the basis that high resolution and high accuracy of the DNA high-pass sequencing detection data are fully played.

Description

technical field [0001] The invention belongs to the field of molecular biological information detection. Specifically, it is a correction method for improving the accuracy of DNA high-throughput sequencing gene expression detection data. Background technique [0002] With the advancement of information science experiment technology, the experimental methods used to obtain molecular biological information are also changing with each passing day. Among them, as an epoch-making molecular biological information detection technology, DNA high-pass sequencing technology has the ability to truly realize high-resolution and high-precision detection of gene expression information of the whole genome. [0003] The gene expression detection principle of DNA high-pass sequencing technology is to directly sequence the target nucleotide sequence reflecting gene expression, and then find the position of the target nucleotide sequence in the reference genome through sequence alignment mapp...

AI Technical Summary

Problems solved by technology

That is, part of the sequencing results cannot be successfully mapped back

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