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Method and composition for restoration of age-related tissue loss in the face or selected areas of the body

A composition and regional technology, applied in the direction of drug combination, pharmaceutical formula, cosmetic preparations, etc., can solve the problem of limited long-term effect of tissue restoration

Inactive Publication Date: 2012-08-01
NEOSTEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Although in the past 50 years, various injectables have been developed and used clinically to restore age-related tissue loss in the face, due to various limitations in materials or compatibility with tissues, the role in restoring tissue loss is limited. limited long-term effect

Method used

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  • Method and composition for restoration of age-related tissue loss in the face or selected areas of the body
  • Method and composition for restoration of age-related tissue loss in the face or selected areas of the body
  • Method and composition for restoration of age-related tissue loss in the face or selected areas of the body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1: Protocol for Skin Renewal Using Autologous Adult Stem Cells

[0126] Clean the skin with 70% ethanol. Then give to Emed, Inc. SilkPeel TM Treated to remove superficial skin barrier and total bacteria. The skin is then vacuumed using a standard dermatological vacuum set on high. The skin was then exposed to Omnilux blu light (wavelength 415nm) for 15 minutes to kill remaining bacteria. This is followed by exposing the skin to Omnilux red light (wavelength 633nm) to open the skin's pores and expand the subcutaneous reticular tissue to improve stem cell survival.

[0127] Use 70% ethanol for a second skin cleansing. At this time, the previously harvested autologous stem cells in 2 vials with approximately 5,000,000 cells each were removed from the liquid nitrogen and placed in warm water. Centrifuge cells for 5 minutes once thawed to allow cells to pellet. The DMSO in which the cells had been frozen was removed using a small syringe. Stem cells were suspe...

Embodiment 2

[0133] Example 2: A screening study examining the effect of two specific growth factor formulations on the lipogenesis of human mesenchymal stem cells

[0134] A screening approach was performed to assess the adipogenic potential of two different growth factor formulations (GFF-I, GFD-I) on human bone marrow-derived mesenchymal stem cells. When used in vivo, the growth factor formulation is embedded in a hyaluronic acid gel matrix to allow long-term (3 months) release of the growth factor matrix.

[0135] Because the purpose was to screen the effect of each growth factor formulation individually, they were transferred using sterile pipettes to separate culture plates containing human mesenchymal stem cells.

[0136] Mesenchymal stem cells were seeded in 6-well tissue culture plates at 3 different concentrations: 10 3 , 10 4 and 10 5 μl.

[0137] result

[0138] After 1 day, check the cells for fat globules. Such as figure 1As depicted in the upper (rt) and (lt) panels, ...

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Abstract

This application relates to stem cell compositions and methods for restoring age-related tissue loss in the face and other selected areas of the body. In a first embodiment, a composition includes stem cells and hyaluronic acid as a carrier wherein the stem cells are peripheral blood stem cells, bone marrow-derived blood stem cells, or mesenchymal stem cells.

Description

field of invention [0001] The present invention relates to methods of restoring age-related tissue loss in the face or selected areas of the body. More specifically, this approach injects autologous stem cells that restore age-related tissue loss in selected areas of the face or body. [0002] related application [0003] This application claims the benefit of priority to US Provisional Application No. 61 / 175,275, filed May 4, 2009, which is hereby incorporated by reference in its entirety. Background of the invention [0004] Skin is composed of epidermis and dermis. Underlying these layers is the subcutaneous tissue, which is not normally classified as a layer of the skin. Subcutaneous tissue is also commonly referred to as the subcutaneous fat layer or hypodermis. The outermost epidermis consists of stratified squamous epithelium with an underlying basement membrane. It does not include blood vessels and receives nourishment from the dermis by diffusion. The main ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/02A61K35/28A61K35/35
CPCA61K38/28A61K31/198A61K38/1825A61K35/35A61K2300/00A61K8/982A61Q19/08A61K35/28A61P17/00A61P43/00
Inventor V.贾姆帕帕R.L.史密斯
Owner NEOSTEM
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