New application of ampelopsin sodium in treating bladder cancer
A kind of staphylophyll and bladder cancer technology, applied in the field of medicine, can solve problems such as not suitable for single use, lack of therapeutic drugs and means, and poor tumor suppression effect
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Publication Date
- 2015-03-11
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Abstract
Description
technical field
[0001] The present invention relates to the field of medicaments, and more specifically relates to the use of staphylosin sodium in treating cancers of the urinary system, especially the new application of staphylosin sodium in the treatment of bladder cancer through intravenous administration and the like. Background technique
[0002] Malignant tumors are a great threat to human health and life, and they and cardiovascular diseases have become two major difficulties in medicine. Bladder cancer is the most common malignant tumor of the urinary system, and its incidence rate ranks first among malignant tumors of the urinary system. Currently, bladder cancer is the ninth most common cancer in the world. According to pathological types, bladder cancer can be divided into epithelial tissue and non-epithelial tissue tumors, epithelial tissue tumors account for 95%, and 90% of them are transitional epithelial tumors (transient cell carcinoma, TCC). In foreign cou...
Examples
Embodiment 1
[0077]The Distribution and Urinary Excretion of Staphylocin Sodium in Mice
[0078] The present embodiment uses high-performance liquid chromatography to measure the drug concentration of AMP-Na in serum, urine and tissue after mouse i.v. excretion characteristics.
[0079] ① Preparation of standard curve
[0080] First dissolve the standard (AMP or AMP-Na) in PBS (pH6.0) to prepare a standard solution of known concentration, then mix it with serum, urine, organ tissue (such as liver homogenate), and use the mixed sample Make a standard curve.
[0081] Taking the standard curve of AMP-Na in mouse urine as an example, weigh 0.0231g of AMP-Na preparation (containing AMP-Na 7.2mg), dissolve the drug with 0.125ml of PBS (pH6.0), so that the drug concentration is 57.6mg / ml; take 30 μl of drug and add it to 200 μl NS (normal saline) and mouse blank urine, so that the drug concentration is 7533 μg / ml, then dilute the 57.6 mg / ml drug solution in double or triple order with PBS, ta...
Embodiment 2
[0116] Inhibitory effect of staphylosin sodium on the proliferation of human bladder cancer cells in vitro
[0117] ①Cell lines and drugs
[0118] Human bladder invasive cancer cell line EJ, human bladder transitional epithelial cancer cell line 5637, and human bladder superficial transitional cancer cell line BIU-87 were purchased from the cell bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences. The cell lines were all derived from human primary bladder cancer.
[0119] AMP-Na freeze-dried agent: 180mg / bottle, batch number: 081031, purity 27.8%; special diluent for AMP-Na freeze-dried agent (0.1mol / L, pH=6.8 phosphate buffer): 5ml / bottle, batch number: 081031 , are provided by Guangdong Tahoe Pharmaceutical Technology Co., Ltd.
[0120] Cisplatin (DDP): 10mg / bottle, batch number: 8030031 DB, produced by Jiangsu Qilu Pharmaceutical Co., Ltd.
[0121] ②Experimental method
[0122] The inhibitory effect of AMP-Na on the proliferation of human bladder can...
Embodiment 3
[0169] Study on the effect of intraperitoneal injection of staphylosin sodium in the treatment of transplanted tumors in mouse bladder and orthotopic transplanted tumors of human bladder cancer in nude mice
[0170] ① Drugs and reagents
[0171] AMP-Na, batch number: 100604, specification: 50mg / bottle (lyophilized), provided by Guangdong Taihe Pharmaceutical Technology Co., Ltd., stored at -20°C, diluted with PBS before use. Carboplatin (CBP) for injection (lyophilized type), 0.1g / bottle, product batch number: 0040031.DA.
[0172] ② Animals and cell lines
[0173] Kunming mice (KM), ♀, body weight: 18-22 g, provided by the Medical Experimental Animal Center of Lanzhou University. Nude mice (BALB / c nu / nu), ♀, 6-7w, provided by Hunan Slack Jingda Experimental Animal Co., Ltd. Human bladder cancer EJ cells were purchased from the Cell Bank of Shanghai Institute of Cell Biology, cultured in RPMI 1640 medium (containing 100 U / mL penicillin and 100 μg / mL streptomycin) containing ...