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Multi-sorting-area structure for cell sorting and use method of multi-sorting-area structure

A sorting area and sorting technology, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problems of microchannel failure and inability to sort cells

Inactive Publication Date: 2013-10-09
NORTHWESTERN POLYTECHNICAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is: in order to overcome the shortcoming that when the previous group of sorting microchannels is blocked in the above sorting method, the following microchannels fail to perform cell sorting, the present invention proposes a cell sorting system with multiple sorting areas. The structure of the selection, the blockage of the previous selection area will not affect the subsequent selection area, and it can sort cells of various sizes

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  • Multi-sorting-area structure for cell sorting and use method of multi-sorting-area structure

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specific Embodiment 1

[0029] refer to Figure 1-5 , the multi-selection area structure used for cell sorting in this embodiment is bonded together by three parts: the external interface layer, the PDMS film layer and the base layer; the external interface layer includes cell fluid interfaces at both ends and 2 sets of ventilation interfaces in the middle, The cell fluid interfaces at both ends are cell fluid interface I1 and cell fluid interface II4 respectively, and the two sets of ventilation interfaces in the middle are the first group of ventilation interfaces 2 and the second group of ventilation interfaces 3; there are two indentations formed on the PDMS film layer. The air chambers are respectively air chamber I5 and air chamber II6; the positions of the two air chambers correspond to the positions of the two groups of ventilation interfaces on the external interface layer, and each ventilation interface is connected with the corresponding air chamber; the base layer has Three groups of cell...

specific Embodiment 2

[0039] The multi-selection area structure used for cell sorting in this embodiment is bonded together by three parts: the external interface layer, the PDMS film layer and the base layer; the external interface layer includes cell fluid interfaces at both ends and three groups of ventilation interfaces in the middle; PDMS There are air chambers formed with 3 concaves on the film layer; the position of each air chamber corresponds to the position of each group of ventilation interfaces on the external interface layer, and each ventilation interface communicates with the corresponding air chamber; the base layer has 4 groups of cell sorting areas, each cell sorting area is a thin film formed by indentation, and there are sorting holes with diameters of 10 μm, 15 μm, 20 μm, and 25 μm respectively; the adjacent selection areas of the 4 groups of cell sorting areas pass through 3 The three micro-channels communicate with each other; the three micro-channels correspond to the positio...

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Abstract

The invention discloses a multi-sorting-area structure for cell sorting and a use method of the multi-sorting-area structure, belonging to the field of microfluidic chips. The multi-sorting-area structure consists of an external interface layer, a PDMS (polydimethylsiloxane) thin film layer and a substrate layer which are bonded together, wherein the external interface layer comprises cell sap interfaces at two ends and N groups of ventilation interfaces in the middle; the PDMS thin film is provided with N air chambers which are formed by sinking inwards; the substrate layer is provided with (N+1) groups of cell sorting areas, and each cell sorting area is provided with a sorting hole of which the diameter is di (i=1,2,...,N+1); and adjacent sorting areas are communicated by N miniature pipelines. In the process of cell sorting by the multi-sorting-area structure, as different groups of sorting areas are independent structurally, when the sorting holes in the front group of sorting area are blocked, the back sorting group is not influenced; and by combination of different number of groups of sorting areas, multiple times of sorting on cells with different sizes can be achieved, and (N+1) groups of sorted cells with sizes matching the conditions that d is less than d1, d is more than or equal to d1 and less than d2, ..., and d is more than and equal to dN and less than d(N+1) are obtained.

Description

[0001] Field [0002] The invention relates to a multi-selection area structure for cell sorting and a method for using the same, belonging to the field of microfluidic chips. Background technique [0003] Cell sorting is a necessary means to obtain target cells with uniform properties from complex environments. Cell sorting is a key link in the study of cell physiology and pathology, and it is of great significance for the diagnosis and treatment of major diseases. The research on cell sorting methods and related technologies has always been a research hotspot in the medical field and related fields. With the emergence of microelectromechanical systems (MEMS) technology and its application in the field of biomedicine, many new cell sorting methods have emerged. At present, the traditional cell sorter represented by flow cytometer has the disadvantages of large volume, complex structure and high cost. The microfluidic chip based on MEMS technology can miniaturize the cell so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N5/00
CPCC12M47/04
Inventor 常洪龙陈方璐洪水金寻文鹏
Owner NORTHWESTERN POLYTECHNICAL UNIV
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