Dry powder inhalant of interferon Alpha
A dry powder inhaler and interferon α technology, which is applied in antiviral agents, respiratory diseases, medical preparations with non-active ingredients, etc., can solve the problems of inaccurate control of dosage, dosage discount, and low inhaled dosage
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Embodiment 1
[0039] Embodiment 1: the preparation of the dry powder inhalation particle of interferon alpha
[0040] Prepare each solution for spray drying according to the formula in Table 1 below, and spray dry the solution with a Buchi B-290 spray dryer according to the corresponding spray drying conditions in Table 2 (continue to maintain the inlet temperature and flow rate of the spray drying gas after the spraying of all liquids is completed) 15 minutes) to prepare dry powder inhalation particles of interferon α.
[0041] Table 1 Preparation of the solution formulation for spray drying of the dry powder inhaler granules of interferon α
[0042]
[0043] Table 2 The spray drying conditions of the dry powder inhaler of interferon α
[0044]
Embodiment 2
[0045] Embodiment 2: the quality evaluation of the dry powder inhaler particle of interferon alpha
[0046] According to the provisions of the "Chinese Pharmacopoeia 2010 Edition (Part Three)" appendix "Interferon Biological Activity Assay Method", the activity of interferon α in the solution after the dissolution of the prepared interferon α dry powder inhalation particles of each formula (IU / ml); According to the provisions of the second method Lowry method of "Protein Content Determination Method" in the appendix of "Chinese Pharmacopoeia 2010 Edition (Part Three)", the interferon α in the solution of the prepared interferon α dry powder inhalation particles of each formula was determined after dissolution The concentration (mg / ml) was divided by the two to obtain the specific activity (IU / mg) of interferon α in the prepared interferon α dry powder inhalation particles of each formula. The specific activity of interferon α in each solution for spray drying was measured by ...
Embodiment 3
[0052] Embodiment 3: the dry powder inhaler of interferon alpha is prepared by the dry powder inhaler particle of interferon alpha
[0053] The interferon α dry powder inhalation particle sample of the formula 1-3 obtained according to the method of Example 1 is not mixed with the large particle size carrier, and is directly divided into 20 mg per capsule as the interference of the formula 1'-3' The dry powder inhalation of interferon α is used for the research of embodiment 5; The dry powder inhalation particle sample of the interferon α of formula 4-6 mixes with commercially available large-size lactose carrier granule with the mass ratio of 1:3, and with each capsule The amount of 20 mg was subpackaged and used in the study of Example 5 as a dry powder inhaler of interferon-alpha with formula 4'-6'.
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