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Medium for separating Phytophthora capsici

A technology of Phytophthora capsici and culture medium, which is applied in the field of culture medium for separating Phytophthora capsici, can solve the problems of low separation rate and difficult separation of target bacteria, and achieve the effects of fast speed, simple preparation, practicality, quickness and low cost

Active Publication Date: 2012-11-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the surface disinfection time is too long, the target bacteria will be killed as well as the non-target bacteria, resulting in a low isolation rate of the target bacteria or difficulty in separating the target bacteria.

Method used

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  • Medium for separating Phytophthora capsici
  • Medium for separating Phytophthora capsici
  • Medium for separating Phytophthora capsici

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The inhibitory effect of embodiment 1, four kinds of fungal inhibitors and five kinds of antibiotics to Phytophthora capsici

[0041] 1. Inhibitory effect of four fungal inhibitors on Phytophthora capsici

[0042] Using dimethylformamide as the solvent of carbendazim, using acetone or methanol as the solvent of difenoconazole, prochloraz and pentachloronitrobenzene, prepare 10ml of 5000μg / mL mother solutions of four kinds of agents respectively, four kinds of The weighing amounts of the medicaments were 50.6mg, 52.0mg, 51.5mg and 125.0mg in turn; these four kinds of mother liquors were respectively prepared with series concentrations of carbendazim, pentachloronitrobenzene , prochloraz and difenoconazole drug-containing PDA plate, and the plate with solvent as a control; then the sensitivity of Phytophthora capsici to four fungal inhibitors was determined by the method of mycelial growth rate. The Phytophthora capsici strains to be tested were pre-cultured on a PDA pla...

Embodiment 2

[0051] Embodiment 2, four kinds of antibiotics are to the inhibitory action of bacteria in the environment

[0052] Plants to be tested: Cultivate the susceptible pepper variety in a seedling pot with a height of ф15cm and a height of 12cm, and place it in the pepper experimental field in the Science Park of China Agricultural University for cultivation. After the plant grows to the 6-leaf stage, 30 leaves are collected immediately for the bacterial suspension. preparation.

[0053] Soil for testing: In 2008, soil samples were collected from areas with severe pepper blight in Beizhuangtou Village, Gucheng Town, Dingxing County, Hebei Province, for future use.

[0054] Test method: This test is based on the results of Example 1 to detect whether the selected four concentrations of antibiotics can effectively inhibit the bacteria in the environment. For the detection of bacteria in the environment, it is mainly aimed at bacteria from plant leaves and diseased soil. This is base...

Embodiment 3

[0057] Embodiment 3, be used to isolate the PDA selective culture medium of Phytophthora capsici

[0058] 1. Separation method

[0059] From Beizhuangtou Village, Gucheng Town, Dingxing County, Hebei Province, Hufang Village, Liuzao Town, Dingzhou City, Hebei Province, Yuegezhuang Village, Pinggu Town, Pinggu District, Beijing, and Dasun Village, Dasungezhuang Town, Shunyi District, Beijing in 2007, 2008 and 2009 Disease samples were collected from various villages, Yangling Town, Yangling District, Xianyang City, Shaanxi Province, Linhe District, Bayannur City, Inner Mongolia Autonomous Region, Anhui, Fujian, Tianjin, Xishan, Yunnan, Chenggong, Xinjiang, and Hongshan District, Wuhan , for the isolation of Phytophthora capsici. The incidence of Phytophthora capsici in the field is as follows image 3shown. In the picture, A: Dark green lesions appear at the base of the stem and at the branches of the stem nodes, which are water-stained. Dark brown or dark brown streaks, th...

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Abstract

The invention discloses a medium for separating Phytophthora capsici. The medium is obtained by adding a sterilizing agent 1), 2), 3) or 4 ) as follows to a PDA medium: 1) a sterilizing agent composed of Difenoconazole, carbendazim, quintozene, rifampicin and penicillin; 2) a sterilizing agent composed of Difenoconazole, carbendazim, quintozene, rifampicin and neomycin sulfate; 3) a sterilizing agent composed of Difenoconazole, Prochloraz, quintozene, rifampicin and neomycin sulfate; and 4) a sterilizing agent composed of carbendazim, quintozene, rifampicin and penicillin. Experiments have proved that the PDA selective medium provided by the invention of has characteristics of feasibility, accuracy, utility, efficiency, low cost, and simple preparation. The PDA selective medium provided by the invention can be used to separate Phytophthora capsici to solve problems of severe pollution caused by non-target fungi and bacteria in a separation process of plant pathogen oomycetes, like Phytophthora capsici, and has advantages of high speed, high efficiency, time saving and labor saving.

Description

technical field [0001] The invention relates to a culture medium for isolating Phytophthora capsici. Background technique [0002] Pepper blight caused by Phytophthora capsici is a worldwide devastating disease. Under suitable climate conditions, it can break out in a short period of time and spread extremely fast. Generally, the onset can cause 30% yield loss, and in severe cases, it can lead to the failure of the pepper harvest. In 1922, Leonian first reported the discovery of Phytophthora capsici disease at the Las Cruces Agricultural Experiment Station in New Mexico, which has now commonly occurred all over the world, causing serious economic losses to agricultural production. In my country, the disease was first discovered in Jiangsu in the 1960s by Yu Dafu. After the 1980s, the disease occurred in more than 20 provinces and cities such as Xinjiang, Gansu, Beijing and Yunnan. Pepper epidemics have occurred in provinces (cities) such as China, Shanghai, and Shaanxi, and...

Claims

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Application Information

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IPC IPC(8): A01N47/18A01N43/90A01N43/653A01N33/18A01P3/00C12N1/14C12R1/645
Inventor 刘西莉毕扬王茜陈磊卢晓红胡健李健强刘鹏飞
Owner CHINA AGRI UNIV
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