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Diamondback moth extract and application thereof

A technology of diamondback moth and extract, which is applied in the direction of drug combination, medical preparations containing active ingredients, medical raw materials derived from arthropods, etc., can solve the heavy economic burden on patients and families, the increase in the number of patients, and the high cost of treatment, etc. problem, to achieve the effect of not easy bacterial resistance, less toxic and side effects, and bacterial resistance

Active Publication Date: 2014-11-19
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment methods for liver cancer mainly include surgical treatment, radiofrequency ablation, microwave ablation, high-intensity focused ultrasound, arterial chemoembolization, alcohol injection, cryotherapy, radiotherapy, molecular targeted therapy, immunotherapy and traditional Chinese medicine treatment. Patients and families have brought a heavy economic burden, and there is currently no effective treatment, and the overall number of patients will show an upward trend. Liver cancer will still be one of the diseases that endanger people's health

Method used

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  • Diamondback moth extract and application thereof
  • Diamondback moth extract and application thereof
  • Diamondback moth extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 : Preparation of diamondback moth extract:

[0032] (1), obliquely puncture the abdominal cavity of the diamondback moth larvae with a small needle with Escherichia coli, stimulate and induce the production of antimicrobial peptides, and continue to raise for 24 hours after the stabbing;

[0033] (2), the insect saline that diamondback moth is added by weight to volume ratio is 1g:3ml, the concentration of each substance in described insect saline is phenylmethylsulfonyl fluoride 10 μ g / ml, NaCl 130mM, KCl 5mM and CaCl 2 1mM, fully mash the homogenate with a homogenizer, add ammonium acetate buffer to the homogenate, centrifuge at 15000r / min, 4°C for 20min, and collect the supernatant;

[0034] (3) Filtrating the supernatant obtained in step (2), back-filtering the initial filtrate, and freeze-drying the filtrate to obtain the extract of Plutella xylostella.

Embodiment 2

[0035] Example 2 : Preparation of diamondback moth extract:

[0036] The steps of this embodiment are the same as those in Example 1, the difference being that the small needle with Staphylococcus aureus is used to puncture the abdominal cavity of the larvae of Plutella xylostella larvae obliquely, continue to raise for 36h, add the insect saline to extract the diamondback moth by weight to volume ratio 1g:4ml , homogenate, centrifuge, filter, and freeze-dry to obtain the extract of diamondback moth.

Embodiment 3

[0037] Example 3 : Preparation of diamondback moth extract:

[0038] The steps of this embodiment are the same as those in Example 1, the difference is that the abdominal cavity of Plutella xylostella larvae is stabbed obliquely with a sterile small needle, and continued to be raised for 48h, and the plutella xylostella is extracted by weight to volume ratio 1g: 5ml, and the insect physiological saline is extracted, homogenized, Centrifuge, filter, and freeze-dry to obtain the extract of diamondback moth.

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Abstract

The invention, a diamondback moth extract and applications thereof, belongs to the technical fields of traditional Chinese medicine preparation and preparation method thereof. The diamondback moth extract is prepared by the method which comprises the following steps of: (1) stimulating and inducing the generation of antibacterial peptide by using a small bacteria-carrying needle to stab obliquely into the abdominal cavity of a diamondback moth, and proceeding to breeding the stabbed diamondback moth for 24 hours; (2) extracting from the diamondback moths in insect normal saline, triturating fully by a homogeneous machine for homogenate, adding ammonium acetate buffer into the homogenate, centrifuging and collecting supernatant; (3) coarse filtering the supernatant obtained in step (2), fine filtering the coarse filtrate, freeze drying the obtained filtrate, and obtaining the diamondback moth extract with the antibacterial peptide content of 1.5%-1.8%. The antibacterial peptide-containing bulk drug extracted from the diamondback moth is delivered through parenteral and gastrointestinal tract paths for antibiosis and antineoplastic treatment, and has the characteristics of simple extraction technology, low cost and good curative effect.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparations and preparation methods thereof. It specifically relates to the extract of diamondback moth and its application. Background technique [0002] Trauma infection is a common disease caused by pathogenic microorganisms and parasites infecting local wounds. There are many kinds of factors causing infection, and the treatment cycle for trauma infection is generally long, which brings a lot of inconvenience to patients. At present, the drugs for the treatment of traumatic infections are mainly concentrated in antibiotics, and the treatment process is often accompanied by side effects, such as drug toxicity, allergic reactions, superinfection, and the emergence of bacterial drug resistance. Antimicrobial peptides have very obvious advantages in the treatment of such diseases. [0003] Liver cancer refers to malignant tumors that occur in the liver, including primary l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61K35/64A61P31/00A61P35/00
CPCY02A50/30
Inventor 袁海龙韩晋徐和徐平华李仙义靳士晓靳世英孟小林
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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