Method for preventing daemonorops margaritae callus browning phenomena from occurring

A technology of callus and yellow vine, which is applied in the field of preventing the browning of yellow vine callus, and can solve problems such as low rooting survival rate, unsatisfactory effect, and difficulty in collecting seeds

Inactive Publication Date: 2012-12-05
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the characteristics of secondary fruiting of vine seeds, the time requirement for collection is very strong, so it is difficult to collect seeds, and it is difficult to meet production needs with seed propagation
Although there are also research reports on cutting propagation, the effect is not very satisfactory, the rooting time is long, and the rooting survival rate is low

Method used

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  • Method for preventing daemonorops margaritae callus browning phenomena from occurring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 (3 days turn bottle)

[0019] The fresh yellow vine young and tender stems with terminal buds collected in May are stored in the refrigerator for 3 days;

[0020] The stems with terminal buds treated in step were first disinfected with alcohol, then treated with mercuric chloride for 10 minutes, and then inoculated on the primary culture medium;

[0021] The stem segment inoculated in step is firstly cultured in dark, and after 3 days of dark culture, it is transferred to light culture, and then transferred to liquid medium after one week; after 3 days of culture in liquid medium, it is transferred to solid medium, and the solid medium is cultivated. After 3 days, transfer to the liquid medium, and transfer the liquid and solid medium to the bottle once every 3 days according to the law, and the duration is 27 days.

Embodiment 2

[0022] Embodiment 2 (2 days turn bottle)

[0023] The fresh yellow vine young and tender stems with terminal buds collected in June were stored in the refrigerator for 2 days;

[0024] The stems with terminal buds treated in step were first disinfected with alcohol, then treated with mercuric chloride for 10 minutes, and then inoculated on the primary culture medium;

[0025] The stem segment inoculated in step is firstly cultured in dark, and after 3 days of dark culture, it is transferred to light culture, and then transferred to liquid medium after one week; after being cultivated in liquid medium for 2 days, it is transferred to solid medium, and the solid medium is cultivated. After 2 days, transfer to the liquid medium, and transfer the liquid and solid medium to the bottle once every 2 days according to the law, and the duration is 28 days.

Embodiment 3

[0026] Embodiment 3 (7 days turn bottle)

[0027] The fresh yellow vine young and tender stems with terminal buds collected in May are stored in the refrigerator for 3 days;

[0028] The stems with terminal buds treated in step were first disinfected with alcohol, then treated with mercuric chloride for 10 minutes, and then inoculated on the primary culture medium;

[0029] The stem segment inoculated in step is firstly cultured in dark, and after 3 days of dark culture, it is transferred to light culture, and then transferred to liquid medium after one week; after 7 days of culture in liquid medium, it is transferred to solid medium, and the solid medium is cultivated. After 7 days, transfer to the liquid culture medium, and transfer the liquid and solid medium to the bottle once every 7 days according to the law, and the duration is 28 days.

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Abstract

The invention discloses a method for preventing daemonorops margaritae callus browning phenomena from occurring. Through preserving young and tender stem sections with terminal buds of fresh daemonorops margaritae in a refrigerated manner, and disinfecting and sterilizing the stem sections by using alcohol and mercuric chloride before the stem sections are inoculated, utilizing middle-later stage combined culture of callus culture and continuously spinning bottles, the daemonorops margaritae browning phenomena are effectively prevented or even eliminated, the browning rate is reduced, the induction rate of the daemonorops margaritae is increased to a certain degree, the growth of plant is promoted and rapid breeding of daemonorops margaritae seedlings is guaranteed, so sa to effectively guarantee the supply of the daemonorops margaritae seedlings and provide support for industrialization development of the daemonorops margaritae.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for preventing the browning phenomenon of the yellow vine callus. Background technique [0002] Huangteng is the cane of Fibraurea recisa Pierre, a plant belonging to the genus Fibraurea recisa Pierre in the family Fanggaeceae. Huangteng is mainly distributed in Guangxi and Yunnan in my country, and also in Vietnam, Myanmar and other countries. Huangteng not only has the effect of clearing heat, detoxifying and diuresis, but also prevents meningitis and lowers blood pressure. It is mainly used for gynecological inflammation, surgical infection, bacillary dysentery, enteritis, respiratory infection and conjunctivitis. The main raw material of Chinese patent medicines such as Huangtengsu Injection, Huangtengsu and Huangtengsu Tablets. With the development of new drugs such as Huangtengsu suppository, the demand for Huangteng resources is increasing day by day, resulting i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 闫志刚胡东南冯世鑫徐永莉栗东霞
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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