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Method for preventing daemonorops margaritae callus browning phenomena from occurring

A technology of callus and yellow vine, which is applied in the field of preventing yellow vine callus browning, and can solve problems such as unsatisfactory effects, affecting the growth and development of yellow vine tissue culture seedlings, and low rooting survival rate

Inactive Publication Date: 2014-03-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the characteristics of secondary fruiting of vine seeds, the time requirement for collection is very strong, so it is difficult to collect seeds, and it is difficult to meet production needs with seed propagation
Although there are also research reports on cutting propagation, the effect is not very satisfactory, the rooting time is long, and the rooting survival rate is low, which limits the development of this tree species in my country to a certain extent.
Tissue culture is the only method that can rapidly reproduce seedlings without being limited by external factors such as seasons. However, browning pollution and other reasons will seriously affect the growth and development of Huangteng tissue culture seedlings, which needs to be solved urgently

Method used

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  • Method for preventing daemonorops margaritae callus browning phenomena from occurring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 (3 days spin bottle)

[0019] Store the young stems with top buds of fresh yellow vines collected in May in the refrigerator for 3 days;

[0020] step The treated stems with apical buds are first disinfected with alcohol, and then treated with mercury for 10 minutes, and then inoculated on the primary culture medium;

[0021] step The inoculated stems are cultured in dark first, dark cultured for 3 days and then converted to light culture, and then transferred to liquid culture medium after one week; liquid culture medium is cultured for 3 days and then transferred to solid medium, solid culture medium is cultured for 3 days and then transferred to liquid culture According to the law, the liquid and solid medium are rotated once every 3 days, and the duration is 27 days.

Embodiment 2

[0022] Example 2 (2 days spin bottle)

[0023] Store the young stems with top buds of fresh yellow vine collected in June in the refrigerator for 2 days;

[0024] step The treated stems with apical buds are first disinfected with alcohol, and then treated with mercury for 10 minutes, and then inoculated on the primary culture medium;

[0025] step The inoculated stems are cultured in the dark first, dark cultured for 3 days, then converted to light culture, and then transferred to liquid culture medium after one week; the liquid culture medium is cultured for 2 days and then transferred to solid media, and the solid culture medium is cultured for 2 days and then transferred to liquid culture According to the law, the liquid and solid medium are rotated once every 2 days, and the duration is 28 days.

Embodiment 3

[0026] Example 3 (Rotating bottle in 7 days)

[0027] Store the young stems with top buds of fresh yellow vines collected in May in the refrigerator for 3 days;

[0028] step The treated stems with apical buds are first disinfected with alcohol, and then treated with mercury for 10 minutes, and then inoculated on the primary culture medium;

[0029] step The inoculated stems are cultured in dark first, dark cultured for 3 days and then converted to light culture, and then transferred to liquid medium after one week; liquid medium is cultured for 7 days and then transferred to solid medium, solid medium is cultured for 7 days and then transferred to liquid culture According to the law, the liquid and solid medium are rotated once every 7 days, and the duration is 28 days.

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Abstract

The invention discloses a method for preventing daemonorops margaritae callus browning phenomena from occurring. Through preserving young and tender stem sections with terminal buds of fresh daemonorops margaritae in a refrigerated manner, and disinfecting and sterilizing the stem sections by using alcohol and mercuric chloride before the stem sections are inoculated, utilizing middle-later stage combined culture of callus culture and continuously spinning bottles, the daemonorops margaritae browning phenomena are effectively prevented or even eliminated, the browning rate is reduced, the induction rate of the daemonorops margaritae is increased to a certain degree, the growth of plant is promoted and rapid breeding of daemonorops margaritae seedlings is guaranteed, so sa to effectively guarantee the supply of the daemonorops margaritae seedlings and provide support for industrialization development of the daemonorops margaritae.

Description

Technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for preventing yellow vine callus browning. Background technique [0002] Yellow cane is the cane of Fibraurea recisa Pierre (Fibraurea recisa Pierre), also known as Garcinia Cambogia and Rhizoma Sinensis. It was included in the 2005 edition of "The Pharmacopoeia of the People's Republic of China". Yellow rattan is mainly distributed in Guangxi and Yunnan of our country, in addition to Vietnam, Myanmar and other countries. Huangteng not only has the effects of clearing heat, detoxifying, and diuretic, but also preventing meningitis and lowering blood pressure. It is mainly used for gynecological inflammation, surgical infection, bacillary dysentery, enteritis, respiratory infection and conjunctivitis. It is a yellow rattan suppository, The main raw materials of Chinese patent medicines such as yellow rattan injections, yellow rattan and yellow rattan tablets. With the develo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 闫志刚胡东南冯世鑫徐永莉栗东霞
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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