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Kit for separating and identifying shigella as well as preparation and application for kit

A Shigella and kit technology, applied in Shigella isolation and identification kits, preparation and application fields, can solve the problems of high cost, complicated identification steps, high missed detection rate, etc.

Inactive Publication Date: 2012-12-12
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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Problems solved by technology

It mainly solves the technical problems of high missed detection rate, complicated identification steps and high cost, and unintuitive screening results existing in the existing Shigella isolation methods

Method used

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  • Kit for separating and identifying shigella as well as preparation and application for kit

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Experimental program
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Embodiment Construction

[0049] First, kits are prepared according to the content of the technical plan, and users of different laboratories (clinical laboratories and public health laboratories) choose to use step-by-step enrichment or direct selective plate for separation according to different samples, and finally use simple biochemical and specific Sexual enzyme-substrate reaction test for differential diagnosis of groups A-D Shigella (Table 2).

[0050] 1. Collection and processing requirements and testing methods of different test materials in clinical laboratories

[0051] 1) Feces: collect as fresh as possible about 1g (thumb size) of human (patients and carriers) feces, and about 1ml of liquid feces, and culture them within 1h-2h. If it cannot be detected for a short time, it should be stored in a commercial medium and shipped to the laboratory within 24 hours. First use a cotton swab (swab) to pick up a stool sample and directly inoculate it on a xylose lysine deoxycholate agar plate (XLD),...

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Abstract

The invention aims at a kit for shigella in enteric pathogenic bacterias as well as a use method for the kit, and mainly solves technical problems of high omission factor, complex identifying steps, higher cost and insufficiently visualized screened results in the existing method for separating the shigella. The kit comprises a universal type nonselective enrichment broth, a 10-time concentrated universal type nonselective enrichment broth, a shigella enrichment broth, a xylose lysine deoxycholate agar plate, an enzyme developing agar plate, a shigella biochemical identification tube, a hydrogen sulfide filter paper strip and an indole filter paper strip, and is characterized by further comprising liquid paraffin oil. All shigellas are identified through a reaction combination test of a substrate and a typical colonial morphologic, simple biochemical and specific enzyme correspondingly grown in a culture medium for selective separation.

Description

technical field [0001] The present invention is a kit and a use method for Shigella among enteropathogenic bacteria, and particularly relates to Shigella (including A group dysentery Shiga, B group Shiga flexneri, C group Bowman's Shiga, D group Song The simultaneous isolation and identification kit, preparation and application of Shiga within Shiga), using the typical colony morphology corresponding to the growth of the selective isolation medium, simple biochemical and specific enzyme-substrate reaction combination tests to identify all Shigella. Background technique [0002] The genus Shigella is currently divided into 4 groups, the common ones are Shigella flexneri and Shigella sonnei, because of its genetics, biological characteristics and the presence of most non-pathogenic bacteria or conditional pathogens in many Enterobacteriaceae biological Due to the similarity of genetics and genetics, many laboratories cannot achieve the expected effect of isolating all Shigella...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/01
Inventor 许学斌袁政安金汇明
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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