Natural antioxidant detecting method

A natural antioxidant and detection method technology, applied in the direction of material electrochemical variables, etc., can solve the problems of high cost, complicated operation, low sensitivity, etc., and achieve the effect of long oscillation life, simple operation and high stability

CN102818827BInactive Publication Date: 2014-07-09ANHUI UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2014-07-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a natural antioxidant detecting method, which is characterized in that a KIO3-MA-H2O2-H2SO4-[NiL](ClO4)2 nonlinear oscillation system is used as detection liquid, a work curve is built for the oscillatory response of the solution on natural antioxidants, further, the qualitative or quantitative analysis on the natural antioxidants is realized, L in the [NiL](ClO4)2 is 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene, and all ingredients in the detection solution have the following mol concentrations that potassium iodate is 0.007 to 0.0735 mol / L, malonic acid is 0.025 to 0.375 mol / L, sulfuric acid is 0.00625 to 0.375 mol / L, hydrogen peroxide is 0.6 to 1.4 mol / L, and [NiL](ClO4)2 is 20.217 to 7.26 mol / L. The method has the advantages that the oscillation service life of the nonlinear system is long, the stability is high, specificity and high sensitivity are realized on the natural antioxidants, the quantitative analysis can be realized, meanwhile, the qualitative analysis can be carried out, in addition, the operation is simple, and convenience and high speed are realized.
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Description

1. Technical field

[0001] The present invention relates to a kind of analysis method of natural substance, relate in particular to the analysis method of natural antioxidant in food, specifically a kind of nickel complex [NiL](ClO 4 ) 2 Catalytic nonlinear systems for the detection of natural antioxidants. 2. Background technology

[0002] With the advancement of science and technology, people have gradually discovered that the natural antioxidants extracted from plants have a good effect in anti-oxidation, and they are used in food, health care, medical treatment, cosmetics production and other fields. Antioxidants play an antioxidative role by interfering with the chain initiation and chain growth process in the free radical chain reaction and eliminating active oxygen. People usually add antioxidants to food, cosmetics or health care products to protect food from corrosion, improve the shelf life of cosmetics or enhance human immunity, etc. However, some synthetic anti...

Examples

Embodiment 1

[0053] Applying KIOs 3 -MA (malonate)-H 2 o 2 -H 2 SO 4 -[NiL](ClO 4 ) 2 The Briggs-Rauscher oscillation system was used as the detection solution for quantitative analysis of ascorbic acid. Add different contents of ascorbic acid, establish a working curve (such as a linear relationship diagram) between the concentration of antioxidant (ascorbic acid) and the change value of the oscillation parameter, and realize the detection of the content of antioxidant ascorbic acid.

[0054] (1) Prepare the solution

[0055] First prepare the sulfuric acid solution of 0.025mol / L with 98% concentrated sulfuric acid as a solvent; then use the sulfuric acid solvent of 0.025mol / L to prepare respectively the potassium iodate solution of 0.14mol / L, the malonic acid solution of 2.0mol / L, and the 4.0 mol / L hydrogen peroxide solution and 0.0173 [NiL](ClO 4 ) 2 solution. Then, add 15ml, 0.025mol / L sulfuric acid solution to the open system of the 50ml beaker; 6ml, 0.14mol / L potassium ioda...

Embodiment 2

[0061] Applying KIOs 3 -MA (malonate)-H 2 o 2 -H 2 SO 4 -[NiL](ClO 4 ) 2 The Briggs-Rauscher oscillation system was used as the detection solution for quantitative measurement of phytic acid. By adding different contents of phytic acid, a working curve (such as a linear relationship diagram) is established between the concentration of antioxidant (phytic acid) and the change value of the oscillation parameter, so as to realize the function of indirectly detecting the content of antioxidant phytic acid.

[0062] (1) Prepare the solution

[0063] First prepare the sulfuric acid solution of 0.025mol / L with 98% concentrated sulfuric acid as a solvent; then use the sulfuric acid solvent of 0.025mol / L to prepare respectively the potassium iodate solution of 0.14mol / L, the malonic acid solution of 2.0mol / L, and the 4.0 mol / L hydrogen peroxide solution and 0.0173 [NiL](ClO 4 ) 2 solution. Then, add 15ml, 0.025mol / L sulfuric acid solution to the open system of the 50ml beaker...

Embodiment 3

[0069] Applying KIOs 3 -MA (malonate)-H 2 o 2 -H 2 SO 4 -[NiL](ClO 4 ) 2 The Briggs-Rauscher oscillation system was used as the detection solution for quantitative measurement of rutin. Add different contents of rutin, and establish a working curve (such as a linear relationship graph) between the concentration of antioxidants and the change value of the oscillation parameter. Realize the function of indirectly detecting the content of antioxidant rutin.

[0070] (1) Prepare the solution

[0071] First prepare the sulfuric acid solution of 0.025mol / L with 98% concentrated sulfuric acid as a solvent; then use the sulfuric acid solvent of 0.025mol / L to prepare respectively the potassium iodate solution of 0.14mol / L, the malonic acid solution of 2.0mol / L, and the 4.0 mol / L hydrogen peroxide solution and 0.0173 [NiL](ClO 4 ) 2 solution. Then, add 15ml, 0.025mol / L sulfuric acid solution to the open system of the 50ml beaker; 6ml, 0.14mol / L potassium iodate solution; 3ml,...