Method for fermentation production of epsilon-polylysine by corn soaking water

A technology of corn soaking water and polylysine, applied in the field of fermentation engineering, can solve the problems of low utilization rate of corn soaking water, achieve the effects of protecting the environment, increasing yield and sugar-acid conversion rate

Inactive Publication Date: 2012-12-19
JILIN COFCO BIOCHEM
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The invention provides a method for fermenting corn soaking water to produce ε-polylysine to solve the problem of low utilization rate of corn soaking water

Method used

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  • Method for fermentation production of epsilon-polylysine by corn soaking water
  • Method for fermentation production of epsilon-polylysine by corn soaking water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Precipitation: Let the fresh corn soaking water stand for 24 hours or filter to remove solids;

[0019] (2) Ingredients: Add the components of the medium into the corn soaking water treated in (1), glucose 40 g / L, yeast extract 3 g / L, KH 2 PO 4 1.5 g / L, K 2 HPO 4 1 g / L, (NH 4 ) 2 SO 4 8 g / L, the volume ratio of tap water and corn soaking water is 1:1, as feed solution, adjust pH to 3.9-4.1; as culture solution, adjust pH to 6.8-7.2;

[0020] (3) Sterilization: maintain the prepared culture at 121°C for 15 minutes, then lower it to 26°C;

[0021] (4) Inoculation: Inoculate according to the inoculum amount of 10% (v / v). The Streptomyces virginiae used has been preserved by the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation number is CGMCC No.6420, and the preservation date is As of August 13, 2012, the address of the preservation unit: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing;...

Embodiment 2

[0024] (1) Precipitation: Let the fresh corn soaking water stand for 24 hours or filter to remove solids;

[0025] (2) Ingredients: Add the components of the medium into the corn soaking water treated in (1), glucose 45 g / L, yeast extract 4.5 g / L, KH 2 PO 4 1.5 g / L, K 2 HPO 4 1 g / L, (NH 4 ) 2 SO 4 10 g / L, the volume ratio of tap water and corn soaking water is 1:1, as feed solution, adjust pH to 3.9-4.1; as culture solution, adjust pH to 6.8-7.2;

[0026] (3) Sterilization: maintain the prepared culture at 123°C for 18 minutes, and then lower it to 29°C;

[0027] (4) Inoculation: Inoculate according to the inoculum amount of 10% (v / v). The Streptomyces virginiae used has been preserved by the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation number is CGMCC No.6420, and the preservation date is As of August 13, 2012, the address of the preservation unit: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, B...

Embodiment 3

[0030] (1) Precipitation: Let fresh corn soaking water stand for 24 hours or filter to remove solids;

[0031] (2) Ingredients: Add the components of the medium into the corn soaking water treated in (1), glucose 50 g / L, yeast extract 6 g / L, KH 2 PO 4 1.5 g / L, K 2 HPO 4 1 g / L, (NH 4 ) 2 SO 4 12 g / L, the volume ratio of tap water and corn soaking water is 1:1, as feeding solution, adjust pH to 3.9-4.1; as culture solution, adjust pH to 6.8-7.2;

[0032] (3) Sterilization: maintain the prepared culture at 125°C for 20 minutes, then lower it to 32°C;

[0033](4) Inoculation: Inoculate according to the inoculum amount of 10% (v / v). The Streptomyces virginiae used has been preserved by the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation number is CGMCC No.6420, and the preservation date is As of August 13, 2012, the address of the preservation unit: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; ...

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Abstract

The invention relates to a method for fermentation production of epsilon-polylysine by corn soaking water and belongs to the field of fermentation engineering. The corn soaking water is used for replacing partial ingredient water and yeast cream, streptomyces virginiae obtained through screening in the soil is adopted for fermentation production of epsilon-polylysine. The process operation is convenient, the ingredient water can be saved, the energy consumption during concentration of the corn soaking water can also be saved, and meanwhile, the problem of environment pollution generated during the original corn soaking water treatment is also solved. In addition, the acid production rate and the saccharic acid conversion rate are also improved, and the method can be used for industrial-scale fermentation.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and specifically relates to a new method for fermenting and producing ε-polylysine by using corn soaking water instead of part of ingredient water and yeast extract. Background technique [0002] ε-polylysine (ε-PL) is an amino acid homopolymer, which is composed of 25-35 lysine residues linked by α-carboxyl and ε-amino groups. It is easily soluble in water, but insoluble in organic solvents such as ethanol, ether, and ethyl acetate. ε-PL not only for G + and G - It has an inhibitory effect, and it also has a good antibacterial effect on most fungi, and it also has an inhibitory effect on heat-resistant bacillus and some viruses; under certain conditions, it can also significantly inactivate some bacteriophages. It is not affected by pH and is stable to heat (120 °C, 20 min). The US Food and Drug Administration (FDA) approved ε-PL as a generally recognized as safe (GRAS) prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12R1/465
Inventor 王国良金贞花陶进赵雪松曹雪范春艳刘跃芹
Owner JILIN COFCO BIOCHEM
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