Immunochromatography test strip marked by phosphorescent silica nano particles and used for quantitatively detecting cimaterol and preparation method for immunochromatography test strip

An immunochromatographic test paper and silicon dioxide technology, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of decreased sensitivity and selectivity of antigens or antibodies, and achieve image results, high detection sensitivity, and strong photostability Effect

Active Publication Date: 2015-01-21
HENAN ACAD OF AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has the characteristics of high sensitivity and strong specificity, but some fluorescein will produce biological toxicity, resulting in the decrease of sensitivity and selectivity of antigen or antibody

Method used

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  • Immunochromatography test strip marked by phosphorescent silica nano particles and used for quantitatively detecting cimaterol and preparation method for immunochromatography test strip
  • Immunochromatography test strip marked by phosphorescent silica nano particles and used for quantitatively detecting cimaterol and preparation method for immunochromatography test strip
  • Immunochromatography test strip marked by phosphorescent silica nano particles and used for quantitatively detecting cimaterol and preparation method for immunochromatography test strip

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Experimental program
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preparation example Construction

[0042] The preparation process of the test strip of the present invention comprises: the preparation of cimaterol monoclonal or polyclonal antibody, the preparation of the phosphorescent antibody fiber layer, the preparation of the adsorption fiber layer, the preparation of the cellulose film layer and the assembly of the test strip, etc. .

[0043] (1) Preparation of anti-cimaterol monoclonal antibody or polyclonal antibody

[0044] Monoclonal antibody preparation: immunize 6-8 week-old Balb / C mice with 50 μg-100 μg / mouse of cimaterol carrier protein conjugate 3-4 times, with an interval of 3-5 weeks between each immunization, and determine the antibody titer After meeting the requirements, super-immune, and 3 to 4 days later, blood was collected from the infraorbital sinus of the immunized mice, and the positive serum was separated; the neck was dislodged to death, and the body surface of the mice was soaked in 75% alcohol for 5 to 10 minutes to disinfect the body surface, a...

Embodiment 1

[0064] Embodiment one: see figure 2 , image 3 . In the figure, 1 is the supporting layer, which is made of plastic sheet strips, and 2 is the adsorption fiber layer, which is made of glass fiber cotton. The phosphorescent antibody fiber layer 3 is adsorbed with anti-CIM monoclonal antibody phosphorescent antibody glass fiber cotton, and the cellulose film Layer 4 is made of nitrocellulose membrane, and the water-absorbing material layer 5 at the handle end is made of water-absorbing filter paper. Adhesive fiber layer 2, phosphorescent antibody fiber layer 3, cellulose film layer 4, and water-absorbing material layer 5 are pasted and fixed from right to left On the support layer 1, the fibers at the junctions between the layers interpenetrate with each other. On the cellulose membrane layer 4, there is a stealth detection blot 6, which is made of bovine serum albumin solution (BSA) coupled with CIM; a stealth control blot 7 is blotted on the cellulose membrane with a goat a...

Embodiment 2

[0070] Embodiment 2: The structure of the test strip is basically the same as that of Embodiment 1, the difference is that the phosphorescent antibody fiber layer 3 is adsorbed with anti-CIM polyclonal antibodies, the adsorption fiber layer 2 is made of nylon membrane, and the cellulose membrane layer 4 is made of The pure cellulose film, the invisible detection imprint and the invisible control imprint are all "ten", and 8-2 is the blue protective film at the handle end covered on the water-absorbing material layer 5 .

[0071] For the detection of milk samples: Dilute the milk samples with physiological saline to make a 1:2-1:5 suspension of the sample to be tested.

[0072] Operation method: insert the sample end of the CIM test strip into the sample to be tested, the insertion depth does not exceed the marking line, take out the test strip in about 10 seconds, and read the value directly through the fluorescence reader. The value is the fluorescence of the T line and the C ...

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Abstract

The invention relates to an immunochromatography test strip marked by phosphorescent silica nano particles and used for quantitatively detecting cimaterol and a preparation method for the immunochromatography test strip. The test strip comprises a supporting layer, an adsorption layer and a protecting layer, wherein the adsorption layer sequentially comprises an adsorption fiber layer, a phosphorescent antibody fiber layer, a cellulose membrane layer and a water absorbing material layer at a handle end from a testing end; invisible detecting blotting printed by a carrier protein solution of coupling cimaterol and invisible comparison blotting printed by an anti-goat or anti-rabbit mouse IgG antibody solution are arranged on the cellulose membrane layer; the phosphorescent antibody fiber layer is made from glass cellucotton adsorbing phosphorescent antibody; and the phosphorescent antibody is cimaterol antibody marked by the phosphorescent silica nano particles. The test strip is high in specificity and sensitivity, is simple, convenient, visualized and accurate, can be used for quantitative and qualitative detection, can detect pg-scale trace residues to the minimum degree, and is wide in application range, low in cost and easy to popularize and apply.

Description

technical field [0001] The invention relates to an immunochromatographic test paper, in particular to an immunochromatographic test strip marked with phosphorescent silicon dioxide nanoparticles and used for quantitative detection of cimaterol and a preparation method thereof. Background technique [0002] Cimaterol (CIM), also known as cimaterol, cimaterol, belongs to a class of phenethylamine drugs and is a potent β2-receptor agonist. In medicine and veterinary medicine, CIM is mainly used to expand trachea and increase pulmonary ventilation, and can be used for asthma, obstructive pneumonia, smooth muscle spasm and shock. Studies have shown that feeding CIM can increase the feed remuneration of fattening pigs and increase the lean meat rate. It has certain effects on cattle, chickens, ducks, sheep and other animals. Therefore, CIM is often illegally used as a feed additive in the production of animal-derived food . Long-term use may cause CIM to accumulate and remain in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/532
Inventor 职爱民张改平胡骁飞赵东宋春美杨继飞
Owner HENAN ACAD OF AGRI SCI
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