Huperzia serrata endophyte and application thereof

A technology of Huperzia serrata and endophytic bacteria, which is applied in the field of microbial engineering, can solve problems such as not being able to meet the needs of drug sources, and achieve good application prospects

Inactive Publication Date: 2013-02-06
ENSHI QINGJIANG BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the survey, China is the main distribution area of ​​Huperzia serrata wild resources, the natural biomass is 4656.6t, the theoretical recoverable amount is 1604t, and the commercial recoverable amount is no more than 200t. At present, at least 2000t of dried Huperzia serr

Method used

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  • Huperzia serrata endophyte and application thereof
  • Huperzia serrata endophyte and application thereof
  • Huperzia serrata endophyte and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Enrichment and screening of Schizophyllum strains of the present invention

[0027] (1) Collect several whole plants of Hupernia serrata, including stems, leaves, adventitious roots, etc., and wrap them with plastic wrap to keep them fresh.

[0028] (2) Wash the collected fresh Hupercia serrata with tap water to remove the dirt, and then rinse with running water for 30 minutes. After the filter paper blotted the water stains on the surface of the explants, put the explants into a dry jar and move them into the aseptic operation room for operation. In the aseptic operation room, soak the explants with 70% ethanol for 10-15S, rinse with sterile water for 3-4 times, dry the water stains on the surface of the explants with sterile filter paper, and then soak the explants with 0.1% mercuric chloride solution After 15 minutes, rinse with sterile water 3-4 times, and dry the water stains on the surface of the explants with sterile filter paper. After treatment, th...

Embodiment 2

[0033] Embodiment 2: Schizophyllum strain fermentation of the present invention can obtain huperzine A similar compound

[0034] (1) Take the Schizophyllum commune XQYS of the present invention, under aseptic conditions, pick up a small amount of hyphae with an inoculation needle, insert it into a PDA plate, activate and cultivate for 72 hours, and cultivate at a temperature of 28±1°C;

[0035] (2) After 72 hours of activation culture, transfer to PD medium, shake flask culture for 72 hours, culture temperature is 28±1℃;

[0036] (3) The strains obtained after the seeds were fermented for 72 hours were transferred into the improved PD medium and cultured on a shaker for 14 days at a culture temperature of 28±1°C;

[0037] (4) After the fermentation is completed, after the fermentation broth is ultrasonically treated for 30 minutes, the total alkaloids in the fermentation broth are extracted by conventional extraction methods;

[0038] (5) After extracting the total alkaloids, p...

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Abstract

The invention relates to huperzia serrata endophyte and application of the huperzia serrata endophyte. The huperzia serrata endophyte is characterized in that the endophyte is taxonomically named as Schizophyllum commune, named as XQYS, and preserved in the general microbiology centre of China Committee for Culture Collection of Microorganisms at Dec. 5th, 2011; the preservation number is CGMCC No.5510; a rRNA (ribosomal ribonucleic acid) gene sequence of fungus for producing huperzine A includes complete sequences of 18S rRNA segment, ITS1, 5.8S rRNA and ITS2, and a segment of 28S rRNA sequence. Application of the huperzia serrata endophyte in preparation of the huperzine A is provided.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to an endophytic bacterium of Huperz serrata and its application. Background technique [0002] With the rapid increase of the world's aging population, the number of patients with Alzheimer's disease (AD) is increasing, and it has become the third major disease that threatens the lives of the elderly after cardiovascular disease and tumor disease. [0003] Chinese scientist Liu Jiasen et al. in 1986 isolated a new effective monomer of lycopodium alkaloids - Huperzine A (Huperzine A) from the folk herbal medicine Huperzinaserrata (Thumb.). In recent years, a large number of domestic clinical studies have confirmed that huperzine A is a highly efficient, highly selective and reversible acetylcholinesterase inhibitor, which can promote memory reproduction and enhance memory retention. It is effective for AD, vascular dementia, It has a good therapeutic effect on the lea...

Claims

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Application Information

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IPC IPC(8): A01G1/04C12P17/12
Inventor 向极钎帅超群龙澜杨永康覃大吉殷红清李红英程新华李亚杰
Owner ENSHI QINGJIANG BIO ENG
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