Bio-toilet not easy to accumulate dirt
An ecological and toilet technology, applied in the field of sanitation equipment, can solve problems such as easy accumulation of dirt, unpleasant smell, overgrown bacteria, etc., and achieve the effect of wide application area, low cost and realization of automation
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[0032] Example 1 Obtaining of Genetically Engineered Bacteria and Bacteria with Degradation Activity
[0033] We commissioned Beijing Zhongke Longtai Biotechnology Co., Ltd. to conduct sequence analysis on the new xylan gene, and found that its nucleotide sequence is shown in SEQ ID No: 1 in the sequence listing, and the amino acid sequence of the encoded xylanase is shown in Shown in SEQ ID No:2. Then, on the basis of our excellent strain of Saccharomyces cerevisiae ZL-1011 (preservation number is CGMCC No.2018, see Chinese patent 200710142813.4), according to the "Molecular Cloning Experiment Guide" and the operation guide of the commercial reagents used, commissioned It constructs genetically engineered bacteria. In short, the gene encoding the xylanase is as shown in SEQ ID No: 3 of the sequence listing (introduced the Kpn I endonuclease site), reverse primer As shown in SEQ ID No: 4 of the sequence listing (Xba I endonuclease site was introduced), double digestion with...
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