Method for preparing chlorella by high cell density fermentation
A chlorella and cell technology, applied in the biological field, can solve the problems of high output cost, low nutrient content of algae seeds, and difficulty in stabilization, and achieve the effect of short fermentation period
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[0016] Embodiment 1.
[0017] The pre-cultivation is in the L-test tube, adding 5ml of liquid medium in an aseptic operation, using a sterile toothpick to insert the colonies of Chlorella monoalgae on the solid medium, and culturing at 28°C and 140rpm for 24h. Inoculate 5 ml of the pre-culture into a 500 ml Erlenmeyer flask containing 100 ml of liquid medium, and culture with shaking at 150 rpm at 28°C for 24 hours. Inoculate 1600ml Erlenmeyer flask culture fluid into 16 liters of sterilized fermentation broth in a 30-liter photomechanical agitation fermenter, and ferment at 28°C; during the 72-hour fermentation process, use light and low-speed stirring for the first 8 hours. The method of ventilation is carried out, the ventilation volume is: 0.3VVM, and the parallel method of fermentation and cultivation is carried out by light and mechanical agitation and aeration starting from the 9th hour; the ventilation volume is 1.2VVM until 72 hours; the fermentation is until the 24th...
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