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Photo-inactivated viruses and systems and methods of using same

A technology to inactivate and virus, applied in the field of light inactivated microorganism system, can solve the problems of complicated serological diagnosis and so on

Inactive Publication Date: 2013-02-13
GEORGIA STATE UNIV RES FOUND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, serological diagnosis of B virus infection in humans is complicated by the relatively high incidence of immune cross-reactivity with herpes simplex virus infections such as HSV-1 and / or HSV-2

Method used

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  • Photo-inactivated viruses and systems and methods of using same
  • Photo-inactivated viruses and systems and methods of using same
  • Photo-inactivated viruses and systems and methods of using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Modified Photoinactivation Technology Using Psoralen and Broad Spectrum Light Pulses to Inactivate Viruses

[0052] Previous experiments with photoinactivation of viruses included a procedure for "black light" (UVA) irradiation of HVP2 psoralen mixtures in petri dishes. In this procedure, the virus-psoralen mixture is exposed to a "black light" UVA lamp in two sets of 30-minute exposures. The results of this experiment were unsatisfactory because it was time consuming, caused heating and evaporation, and above all resulted in lower antigenicity.

[0053] In order to overcome the shortcomings of the above procedure, this example describes an improved psoralen photoinactivation technique, wherein psoralen photoinactivation was carried out by using Xenon Corporation (Woburn, MA) (Xenon Corporation (Woburn, MA) ) SteriPulse-XL irradiation equipment (RS-3000C type). Information on the SteriPulse-XL system is described in Xenon's publication entitled "Germicidal ...

experiment example 1

[0065] Experimental example 1. The purpose of this experiment was to compare the inactivation process by BSPL with the photoinactivation by the combined use of psoralen and BSPL. Inactivation by BSPL alone was performed as follows: five 1 ml dilutions of HVP2 (10 8 PFU / ml) were transferred to 5 polyethylene tubes (Polytubing, 1 x 1500'2 Mil, product number S-3520, ULINE, Atlanta GA) heat-sealed at one end. Next, heat seal the end at a position 5 cm away from the first seal. First, another 5 ml of diluted virus was mixed with psoralen (4-Aminomethyl-trioxalen hydrochloride, Sigma, Catalog # A43305 mg) to a final concentration of 20 μg / ml psoralen, and then 1 ml was transferred to the above five polyethylene tubes respectively. Each tube was irradiated with different doses of BSPL (with and without psoralen). A pair of polyethylene tubes in each group was placed on a plastic tray lying flat on a bed of crushed ice, one containing the virus and the other containing the virus ...

experiment example 2

[0070] Experimental example 2. The procedure described in Experimental Example 1 was repeated in this Experimental Example with some minor changes. Samples exposed to 3 pulses of BSPL were not spot detected. Spot detection was performed on samples exposed to BSPL pulses of 6 or higher. Table 2 provides the results of spot detection of HVP2 infection by BSPL compared to photoinactivation process in combination with psoralen and PSPL. The number of spots per BSPL dose is shown in bold numbers. All samples exposed to 3 BSPL pulses or higher were detected by PCR ( Figure 4 ). As in Table 2, samples mixed with psoralen and exposed to BSPL pulses of 6 or higher produced no spotting. The samples exposed to BSPL developed spots only after exposure to pulses of 6, 9 and 12, but not after pulse 15.

[0071] Table 2

[0072] Pulse number

6

9

12

15

J / cm 2

2.70

4.05

5.40

6.75

BSPL+Psoralen

0

0

...

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Abstract

The present disclosure relates generally to systems and methods for the photo- inactivation of microorganisms. More specifically, the present invention is directed towards the photo-inactivation of microorganisms, such as viruses, using at least one furanocoumarin and broad spectrum pulsed light. For example, an aspect of the present invention includes a method for inactivating a herpesvirus, such as herpes B virus or herpes virus papio 2 using a psoralen and broad spectrum pulsed light.

Description

[0001] Cross References to Related Applications [0002] Pursuant to 35 U.S.C. $119(e), this application claims the benefit of U.S. Provisional Patent Application No. 61 / 288,756, filed December 21, 2009, the contents of which are hereby incorporated by reference as if fully set forth below. technical field [0003] Various embodiments disclosed herein relate generally to systems and methods for photoinactivating microorganisms. More specifically, various embodiments of the present invention are directed to the photoinactivation of microorganisms, such as viruses, using at least one furanocoumarin compound and broad-spectrum pulsed light. Background technique [0004] Herpes B virus (Herpesvirus simiae or Cercopithecine herpesvirus 1) is a member of the Alphaherpesvirinae subfamily and the Simplexvirus group and is known to occur naturally in rhesus monkeys (Macaca spp). Infection in rhesus monkeys may be asymptomatic, or may cause mild illness. Infection in other species, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/34A61K31/35C12N1/36
CPCC07K16/085A01N43/16A61L2/0076G01N2469/20A61K2039/5252A61L2/0047A61L2202/22A61K39/00G01N33/56994A61P31/00A61P31/22A61P37/04A01N25/00A01N2300/00
Inventor 朱莉亚·赫利尔德大卫·卡兹
Owner GEORGIA STATE UNIV RES FOUND INC
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