Method for preparing extract with over 50% of picrorhiza saponin II

A technology of Coptis chinensis saponins and extracts, which is applied in the field of preparation of Coptis chinensis saponin II greater than 50%, which can solve the problems of acute poisoning, easy volatility, high cost, etc., and achieve high anti-hepatitis B virus and choleretic pharmacological activities , The effect of safety improvement

Inactive Publication Date: 2013-02-27
JINAN KANGZHONG PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There have been many literature reports and many patents on the method of extracting and preparing the total saponins of berberine from berberine. The preparation method, according to the basic skeleton of the macroporous resin, the properties of the filler and the porogen, may contain residues such as alkanes, benzene, toluene, xylene, styrene, divinylbenzene, naphthalene, etc. in the macroporous resin, use The macroporous resin is used for the separation of the adsorbent to prepare the total saponins of Coptis chinensis. It is possible to introduce the residues in the macroporous resin into the product and cause toxicity to the human body. To remove the residues in the macroporous resin, the process is complicated and the cost is high
[0004] Invention patent 200710113065.7, a drug for treating liver damage and its preparation method. The disclosed preparation method of total saponins of Coptis chinensis uses silica gel as an adsorbent to separate and prepare total saponins of Coptis chinensis, but uses a mixture of chloroform and methanol as an eluent Chloroform and methanol are both volatile, inhalation and oral toxicity, or acute poisoning caused by skin absorption, operators must undergo special training and strictly abide by the operating procedures
Operators need to wear filter gas masks, chemical safety protective glasses, anti-static overalls, rubber gloves, etc., which is very inconvenient to operate
And it may remain in the product, causing toxicity
Empty containers of chloroform and methanol may have residues, polluting the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Grind 1 kg of Rhizoma Coptidis, pass through a 24-mesh sieve, and extract twice with ethanol under reflux, each time with 8 kg of 80% ethanol for 1 hour each time, combine the extracts, and filter to obtain the extracts;

[0027] 2) Ethanol is recovered from the extract and concentrated;

[0028] 3) Add 0.5kg of chromatographic silica gel to the concentrated solution, mix well, and dry to obtain the mixture;

[0029] 4) Take 2kg of column chromatography silica gel and use ethanol: ethyl acetate = 1:2 solvent to wet the column to obtain a silica gel column;

[0030] 5) Add the mixture prepared in 3) to the top of the silica gel column, and elute with a solvent of ethanol: ethyl acetate = 1:3. During the elution process, collect a solution containing more than 40% of the total saponins of Coptis chinensis based on the dry product. The solvent is recovered and dried to obtain an extract with a percentage content of coptidis saponin II greater than 50%, and the extract ...

Embodiment 2

[0032] 1) Grind 10kg of Coptis chinensis, pass through a 24-mesh sieve, and extract three times with ethanol under reflux, each time with 100kg of 95% ethanol for 1 hour each time, combine the extracts, and filter to obtain the extracts;

[0033] 2) Ethanol is recovered from the extract and concentrated;

[0034] 3) Add 3kg of chromatographic silica gel to the concentrated solution, mix well, and dry to obtain the mixture;

[0035] 4) Take 20kg of column chromatography silica gel and use ethanol: ethyl acetate = 1:20 solvent to wet the column to obtain a silica gel column;

[0036] 5) Add the mixture prepared in 3) to the top of the silica gel column, and elute with a solvent of ethanol:ethyl acetate=1:20. During the elution process, collect the solution containing more than 50% of the total saponins of Coptis chinensis based on the dry product, recycle the solvent, and dry to obtain 0.6 kg of an extract with a percentage content of Coptis chinensis II greater than 50%. After t...

Embodiment 3

[0038] 1) Crush 5kg of Coptis chinensis, pass through a 10-mesh sieve, and extract 3 times with ethanol reflux, each time with 40kg of 85% ethanol for 1 hour each time, combine the extracts, and filter to obtain the extracts;

[0039] 2) Ethanol is recovered from the extract and concentrated;

[0040] 3) Add 1.25kg of chromatographic silica gel to the concentrated solution, mix well, and dry to obtain the mixture;

[0041] 4) Take 5kg of column chromatography silica gel and use ethanol: ethyl acetate = 1:10 solvent to wet the column to obtain a silica gel column;

[0042] 5) Add the mixture prepared in 3) to the top of the silica gel column, and elute with a solvent of ethanol: ethyl acetate = 1:10. During the elution process, collect a solution containing more than 60% of the total saponins of Coptis chinensis based on the dry product. The solvent was recovered and dried to obtain 0.3 kg of an extract with a percentage content of pidoside Ⅱ greater than 50%, and the extract ...

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PUM

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Abstract

The invention relates to a method for preparing an extract with over 50% of picrorhiza saponin II. The method is that picrorhiza is smashed into coarse powders and then subjected to extraction by using ethanol, silica gel is added for evenly mixing the mixture, then the mixture is dried and placed at the top end of a silica gel column, and the mixture is subjected to elution by using the ethanol, acetic ether and acetone. The method for preparing the picrorhiza saponin II is convenient to operate and high in safety of drug preparation.

Description

technical field [0001] The invention relates to medicines, in particular to a method for preparing an extract containing more than 50% of coptis saponin II and having medicinal uses. Background technique [0002] Huhuanglian is the dried rhizome of Picrorhiza scrophulariiflora Pennell. It is a traditional Chinese medicine. Fever, spontaneous sweating, night sweats, vomiting blood, eye disease, hemorrhoids, sores, etc. The chemical constituents of Huhuanglian mainly include iridoids, cucurbitacins and phenolic glycosides. In recent years, studies at home and abroad have found that the iridoid glycosides (total saponins of Coptis chinensis) in Huhuanglian have various pharmacological activities such as anti-hepatitis B virus, liver protection, and enzyme reduction, and are the main active ingredients. Highest. [0003] There have been many literature reports and many patents on the method of extracting and preparing the total saponins of berberine from berberine. The prepa...

Claims

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Application Information

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IPC IPC(8): C07J17/00
Inventor 李诗标许翠萍张为胜丁桂丽张晶
Owner JINAN KANGZHONG PHARMA TECH DEV
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