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Method for biosynthesizing virginiamycin by streptomycete

A virginiamycin, biosynthesis technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of no domestic manufacturers of virginiamycin, and improve feed utilization. efficiency, process stability and high fermentation yield

Active Publication Date: 2013-02-27
江西兴鼎科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no domestic manufacturer of virginiamycin in the market, and it is mainly imported from Phipo Corporation of the United States.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:

[0035] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;

[0036] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;

[0037] (3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.

[0038] The concentration of each component of the slant nutrient medium is g / L: glucose 2.0, yeast extract 3.0, wort juice 8.0, MgSO 4 0.5, NaCl 0.5, K 2 PO 4 0.5, CaCO 3 2.0, agar 15.0, water balance, medium pH 6.7, culture ...

Embodiment 2

[0043] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:

[0044] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;

[0045] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;

[0046](3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.

[0047] The concentration of each component of the slant nutrient medium is g / L: glucose 3.0, yeast extract 4.5, wort juice 10.0, MgSO 4 0.8, NaCl 0.8, K 2 PO 4 0.8, CaCO 3 3.0, agar 23.0, water balance, medium pH 6.8, culture ...

Embodiment 3

[0052] A method for streptomyces biosynthesis of virginiamycin is achieved by the following measures:

[0053] (1) Slant culture: Inoculate the ACCC40768-Streptomyces virginiana purchased at Beijing Beina Chuanglian Biotechnology Research Institute, the strain preservation number is 40768, on the nutrient medium for slant culture;

[0054] (2) Seed culture: the spores obtained from the slant culture are subjected to seed culture to obtain seed liquid;

[0055] (3) Fermentation culture: Inoculate the seed solution into the fermentation medium for cultivation, and add precursor substances in the early stage of fermentation to obtain a fermentation broth, and obtain the polypeptide antibiotic virginiamycin containing a lactone ring after separation.

[0056] The concentration of each component of the slant nutrient medium is g / L: glucose 4.0, yeast extract 6.0, wort juice 12.0, MgSO 4 1.0, NaCl1.0, K 2 PO 4 1.0, CaCO 3 4.0, agar 30.0, water balance, medium pH 7.0, culture ...

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Abstract

The invention discloses a method for biosynthesizing virginiamycin by streptomycete, which comprises the following steps of: preparing a slope with spores of virginiamycin; inoculating the slope spores into a culture medium to obtain a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation culture, adding a precursor substance at the early stage of fermentation so as to increase the yield of virginiamycin; and further processing the fermentation solution after fermentation to obtain virginiamycin crystals. The method disclosed by the invention is simple and easy to implement, is stable in the fermentation result and low in production cost. The fermentation result is improved compared with the traditional process, and the highest yield of virginiamycin can reach 3500 mu g / ml. Virginiamycin is polypeptide antibiotic containing lactonic rings, has the characteristics of no toxicity, no accumulation, easiness in degradation, and no drug resistance, and is a new animal feed additive.

Description

technical field [0001] The invention relates to a method for biosynthesizing virginiamycin by streptomyces, belonging to the fields of industrial biotechnology and fermentation engineering. Background technique [0002] Virginiamycin ( virginia mycinia ), also known as mastomycin, veromycin, and virgimycin, is produced by Streptomyces virginia ( Streptomyces virginiae ) The chain-positive antibiotic produced by fermentation is a kind of veterinary antibiotic with a relatively novel structure, that is, a polypeptide antibiotic containing a lactone ring. Virginiamycin is structurally composed of two factors: M1 factor (about 70-80%) and S1 factor (about 20-30%). M1 factor is a macrolide with the molecular formula C 28 h 35 N 3 o 7 , especially effective against aureus, S1 factor is a cyclic polypeptide with the molecular formula C 43 h 49 N 7 o 10 , especially effective against Sarcina luteus. These two factors have a synergistic effect. When M:S=70:30, their antib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/465
Inventor 韩风华李国栋邹君彪邓健黄苓
Owner 江西兴鼎科技有限公司
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