Method for detecting ureaplasma urealyticum and tiny urealyticum

Abstract

The invention discloses a method for detecting ureaplasma urealyticum and tiny urealyticum, which belongs to the technical field of detection of microporous plates. The method for detecting ureaplasma urealyticum and tiny urealyticum has the characteristics that clinical samples processed by thermal denaturation after PCR (polymerase chain reaction) amplification are respectively added to micro holes of a DNA-BINDING96 hole determination plate equipped coated with Uu and Up specific probes, and positive control holes are arranged, to which Uu and Up serotype standard strains processed by thermal denaturation after PCR amplification are added; after thermostat hybridization, the plate is cleaned and is patted to be dry; streptavidin-horseradish peroxidase conjugates are added to each hole, the plate is cleaned, and the streptavidin-horseradish peroxidase conjugates are dried in air; then, chromogenic substrates A and B are added to each hole, and color development is performed in a shady place after the mixture becomes homogeneous; finally, a stop solution is added, and the result is determined according to color change. The method for detecting ureaplasma urealyticum and tiny urealyticum not only solves the problem of false positive result caused by determining a positive result according to color change of a liquid medium, but also can accurately identify Uu and Up, thereby improving the detection sensitivity. Moreover, high-flux detection is realized, and the method for detecting ureaplasma urealyticum and tiny urealyticum can be widely used for clinical screening.

Description

technical field [0001] The invention relates to a detection technology of a microporous reaction plate, in particular to a detection method for Ureaplasma urealyticum (Uu) and Ureaplasma parvum (Up). Background technique [0002] Mycoplasma (Mycoplasma) is a class of minimal prokaryotic organisms between bacteria and viruses, without cell walls, able to pass through bacterial filters, and able to grow and reproduce on artificial media. Mycoplasma belongs to Mollicula class, Mycoplasma order, Mycoplasma family. The Mycoplasma family is divided into four genera: Mycoplasma, Bloodworm, Bloodbartonella and Ureaplasma. Among them, a number of studies have proved that Ureaplasma urealyticum is closely related to nongonococcal urethritis, miscarriage, premature birth, and low birth weight infants in the female genitourinary tract. [0003] Janet reported in 1984 that metal manganese ions have an inhibitory effect on Uu. 1mM manganese ions not only inhibited the growth of Ureapla...

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Problems solved by technology

[0007] The present invention aims to solve the problem of false-positive results in the conventional culture method of judging the positive results by the color change of the liquid culture medium, and the problem that Uu and Up cannot be accurately identified, and provides a clinically important urealytic urea Detection method of ureaplasma and ureaplasma micronum

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