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Homocysteine detection kit and preparation method thereof

A technology of homocysteine, detection kit, applied in the field of biology

Inactive Publication Date: 2013-03-13
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports and products on the detection of homocysteine ​​by quantum dot immunochromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 kit

[0030] A quantum dot-labeled homocysteine ​​monoclonal antibody with a particle size of 5 nm, an excitation wavelength of 365 nm, and an emission wavelength of 605 nm was used to obtain a quantum dot-labeled homocysteine ​​monoclonal antibody;

[0031] The sample loading area, the conjugate release area, the test area, the control area and the water absorption area are set in sequence, the conjugate release area is coated with quantum dot-labeled homocysteine ​​monoclonal antibody, and the test area is coated with homocysteine , coating the control area with anti-mouse IgG antibody, preparing detection tools, assembling the kit instruction manual, and obtaining the kit.

Embodiment 2

[0032] The preparation of embodiment 2 kit

[0033] Take quantum dot-labeled homocysteine ​​monoclonal antibody to obtain quantum dot-labeled homocysteine ​​monoclonal antibody;

[0034] The sample loading area, the conjugate release area, the test area, the control area and the water absorption area are set in sequence, the conjugate release area is coated with quantum dot-labeled homocysteine ​​monoclonal antibody, and the test area is coated with homocysteine , coating the control area with anti-mouse IgG antibody, preparing detection tools, assembling the kit instruction manual, and obtaining the kit.

Embodiment 3

[0035] Example 3 Feasibility verification based on the detection method of the kit provided by the invention

[0036] Quantum dot immunochromatography homocysteine ​​quantitative detection kit: the kit provided in Example 1 of the present invention.

[0037] Fluorescence Polarization Immunoassay (FPIA) Homocysteine ​​Quantitative Detection Kit: purchased from Abbott, USA, the main components of the reagent:

[0038] Reagent 1 (15.0m1): s-adenosyl-L-cysteine ​​fluorescein tracer, present in phosphate buffer containing calf protein stabilizer, preservative is sodium azide.

[0039] Reagent 2 (12.4m1): s-adenosyl-L homocysteine ​​hydrolase, present in phosphate buffer containing calf protein stabilizer, preservatives are sodium azide and antibacterial agents.

[0040] Reagent 3 (15.6 ml): trans-s-adenosyl-L-homocysteine, present in phosphate buffer containing calf protein stabilizer, preservative sodium azide.

[0041] Reagent 4 (15.3ml): pretreatment solution, containing citri...

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Abstract

The invention relates to the technical field of biology, in particular to a homocysteine detection kit and a preparation method thereof. According to the method, the traditional colloidal gold is replaced by novel fluorochrome quantum dot, and homocysteine in human serum can be accurately, quantitatively, rapidly and sensitively detected. The instrument required by the kit is different from the previous large expensive instrument in a clinical laboratory, only a small fluorescence quantitative analyzer is required, and real-time detection is realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a homocysteine ​​detection kit and a preparation method thereof. Background technique [0002] Homocysteine ​​(HCY) is a sulfur-containing amino acid and an intermediate metabolite of methionine, which is produced in the body by transmethylation of methionine. In plasma, HCY has 3 forms: HCY, HCY disulfide and HCY-cysteine. In normal human body, there are very few free HCY, about 80% of which are combined with albumin by disulfide bonds, and only a small part is in a free state, all of which are collectively referred to as total HCY. Red blood cells are the main place to produce HCY. During the period of contraction and coagulation after blood is isolated, red blood cells continue to produce HCY and release it into the extracellular fluid: it is generally considered that it is better to measure plasma samples. The currently accepted reference range for determining the normal range ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 马伟民张永顶马新民
Owner SHENZHEN BLOT BIOTECH
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