Preparation method of coix seed polysaccharide and application of coix seed polysaccharide

A polysaccharide and crude polysaccharide technology, applied in the field of nutrition and immunity, can solve the problems of undiscovered patent publications, etc., and achieve the effect of increasing application value, high interest rate and low price

Inactive Publication Date: 2013-03-20
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Through the search, no patent publications related to this patent application were found

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  • Preparation method of coix seed polysaccharide and application of coix seed polysaccharide
  • Preparation method of coix seed polysaccharide and application of coix seed polysaccharide
  • Preparation method of coix seed polysaccharide and application of coix seed polysaccharide

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Embodiment Construction

[0032] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0033] The main idea of ​​the present invention is: using barley as raw material, extract the crude polysaccharide of barley by water extraction and alcohol precipitation method, then obtain the polysaccharide of barley after purification, apply the obtained polysaccharide of barley to non-small cell lung cancer A549 cells, and observe the induction of barley polysaccharide Apoptotic effect of A549 cells.

[0034] In the present invention, coix seed polysaccharides are used to treat human non-small cell lung cancer cells A549 cells. Experiments show that coix seed polysaccharides can produce apoptotic bodies in A549 cells and cause S-phase arrest. Through Annexin V-FITC and PI staining, it is found that coix seed polysaccharides can cause the...

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Abstract

The invention relates to a preparation method of coix seed polysaccharide. The preparation method comprises the following steps of: crushing and screening coix seeds first, and carrying out degreasing, water bath extraction, centrifugation, enzymolysis, concentration, alcohol precipitation and vacuum freeze drying and precipitation to obtain crude coix seed polysaccharide; and dissolving the prepared crude coix seed polysaccharide to obtain a crude coix seed polysaccharide solution, carrying out deproteinization, decoloration, dialysis and ion exchange chromatography on the crude coix seed polysaccharide solution to obtain purified coix seed polysaccharide, and then carrying out vacuum freeze drying to obtain white powder, namely the coix seed polysaccharide. According to the preparation method disclosed by the invention, the crude coix seed polysaccharide is extracted by using a water extraction and alcohol precipitation method, the crude coix seed polysaccharide is subjected to deproteinization, decoloration, dialysis and ion exchange chromatography to obtain the purified coix seed polysaccharide, and then, the purified coix seed polysaccharide is subjected to vacuum freeze drying to obtain the white powder, namely the coix seed polysaccharide; and therefore, the preparation method is simple and the coix seed polysaccharide is high in yield.

Description

technical field [0001] The invention belongs to the field of nutrition and immunity, and relates to the extraction of polysaccharides, in particular to a preparation method and application of barley polysaccharides. Background technique [0002] Barley (Coix), also known as coix seed, rice kernel, coix pearl, bodhi seed, 芑shi, Jieli, etc., is widely planted in warm regions around Asia, Africa and the Mediterranean Sea. It likes to be born in humid areas, but it can tolerate floods and droughts. The dry and mature seed of Job's tears is called Job's tears. Job's tears are solid, white in cross section, powdery, slightly gas, and slightly sweet in taste. Job's tears are baked or processed into Job's tears powder for consumption. The seeds of Job's tears can be used as medicine to treat diseases. Li Shizhen recorded in "Compendium of Materia Medica": Coix seed can "invigorate the spleen and stomach, nourish the lungs and clear heat, remove wind and dampness. Cook food, cure a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 卢相义刘薇罗成李贞景
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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