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Process for production of euglena containing wax ester at high content, and process for production of wax ester

A production method and manufacturing method technology, applied in the direction of biochemical equipment and methods, single-cell algae, fermentation, etc., can solve the problems of low production, food shortage, etc., and achieve the effect of improving environmental problems and increasing energy self-sufficiency

Active Publication Date: 2013-03-27
EUGLENA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Soybeans, corn, palms, etc. are known as higher plants that are candidates for biofuel raw materials, but when edible crops are used as raw materials, food shortages may become a problem
On the other hand, production using non-edible plants such as jatropha and camelina is also advancing, but there is a problem that the production volume per unit area is low

Method used

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  • Process for production of euglena containing wax ester at high content, and process for production of wax ester
  • Process for production of euglena containing wax ester at high content, and process for production of wax ester
  • Process for production of euglena containing wax ester at high content, and process for production of wax ester

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Experimental program
Comparison scheme
Effect test

no. 1 Embodiment approach

[0069] according to figure 1 , the first embodiment of the production method of the euglena genus algae rich in wax esters according to the present invention will be described.

[0070] The production method comprises: step 1 (equivalent to the first step), carrying out aerobic culture of euglena algae with a medium added with a nitrogen source; step 2 (equivalent to the second step), replacing it with a nitrogen source-free medium Carry out aerobic culture in the culture medium; Step 3 (equivalent to the 3rd step), carry out anaerobic treatment, carbohydrate is fermented into wax ester.

[0071] First, the cultivation of the algae of the genus Euglena in step 1 can also be carried out by blowing air into the medium as a carbon dioxide source, but it is preferable to blow carbon dioxide gas into the medium in order to increase the cultivation efficiency.

[0072] That is, since oxygen is contained in the atmosphere or carbon dioxide gas, aerobic culture is performed.

[0073...

no. 2 Embodiment approach

[0093] according to figure 2 , the second embodiment of the production method of the euglena genus algae rich in wax esters according to the present invention will be described.

[0094] It should be noted that, since the medium composition, culture conditions, etc. are the same as those of the first embodiment, the same description will be omitted, and only the differences will be described.

[0095] In the aerobic culture step in Step 1, a medium obtained from the modified Cramer-Myers medium in Step 1 of the first embodiment from which a nitrogen source was removed is used.

[0096]That is, in the initial stage, the aerobic cultivation of the algae of the genus Euglena is started with a medium that does not contain a nitrogen source.

[0097] In step 1, first, in step S1, a nitrogen source is added to the culture medium inoculated with algal bodies of the genus Euglena.

[0098] Then, aerobic cultivation is carried out in step S2.

[0099] Next, in step S3, it is determ...

Embodiment

[0122] (About the production method of euglena algae rich in wax esters)

[0123] Next, the wax ester-rich algae of the genus Euglena according to the present invention will be specifically described by showing an example.

[0124] In this example, Euglena graclilis Z strain was used.

[0125] The medium was modified by modifying Cramer-Myers medium ((NH 4 ) 2 HPO 4 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.2g / L, CaCl 2 2H 2 O 0.02g / L, EDTA·2Na 0.05g / L, Fe 2 (SO 2 ) 3 ·7H 2 O 3mg / L, MnCl 2 4H 2 O 1.8mg / L, CoSO 4 ·7H 2 O 1.5mg / L, ZnSO 4 ·7H 2 O 0.4mg / L, Na 2 MoO 4 2H 2 O 0.2mg / L, CuSO 4 ·5H 2 O 0.02g / L, thiamine hydrochloride (vitamin B 1 ) 0.1mg / L, cyanocobalamin (vitamin B 12 ), (pH5.5)) were prepared by autoclaving.

[0126] (Process 1: Aerobic cultivation)

[0127] After 880 ml of the prepared medium was put into a shaking culture bottle with a capacity of 1 L, the seed algae of Euglena algae were inoculated with an initial concentration of abou...

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Abstract

To provide: a process for producing euglena containing a wax ester at a high content, by culturing a microalga euglena aerobically by photosynthesis using carbon dioxide as a carbon source, further culturing the microalga euglena under nitrogen-starved conditions to increase the amount of accumulated paramylon per cell, and subsequently leaving the microalga euglena under anaerobic conditions, thereby producing euglena containing a wax ester at a high content; and a process for producing a wax ester. [Solution] The invention relates to a process for producing euglena containing a wax ester at a high content. The process comprises a first step of culturing a microalga euglena aerobically, a second step of further culturing the microalga euglena while retaining the culture medium under nitrogen-starved conditions, and a third step of retaining cells of the microalga euglena under anaerobic conditions.

Description

technical field [0001] The present invention relates to a production method of euglena genus rich in wax esters and a method for producing wax esters. The production method of euglena genus rich in wax esters can produce biofuels rich in wax esters at low energy and low cost. Raw material of the wax esters of the microalgae Euglena genus. Background technique [0002] In recent years, the problem of global warming has become increasingly serious, and it has become a major issue to suppress the emission of carbon dioxide gas, which is one of the greenhouse gases, and to reduce the concentration of carbon dioxide in the atmosphere by fixing carbon dioxide. [0003] Under such circumstances, the use of fossil fuels containing fixed carbon dioxide as an energy source causes the fixed carbon dioxide to be released into the atmosphere again, which becomes an environmental problem. In addition, since fossil fuels are limited resources, there is also a problem of depletion. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64
CPCC12N1/12C12N15/8247C12P7/6463C12P7/64C12P7/6436
Inventor 岚田亮莎拉巴妮·米特拉
Owner EUGLENA
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