Brevibacillus borstelensis strain having capability of degrading thioanisole and application thereof

A technology of Brevibacillus brevis and thioanisole is applied in the field of microorganisms, which can solve the problem that Brevibacillus potsdam is not found to degrade thioanisole and the like, and achieve the effect of efficiently degrading thioanisole

Active Publication Date: 2014-08-06
GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Brevibacillus borstelensis strain having capability of degrading thioanisole and application thereof
  • Brevibacillus borstelensis strain having capability of degrading thioanisole and application thereof
  • Brevibacillus borstelensis strain having capability of degrading thioanisole and application thereof

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Embodiment 1

[0021] The Brevibacillus Potsdamer strain capable of degrading sulfide anisole in the present invention is obtained by separating and purifying the bottom mud of a certain river in Guangzhou City, Guangdong Province. Its separation and purification method is as follows: the acclimatization medium used is inorganic salt medium (contain the following components in every liter of inorganic salt medium: K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl 2 ·6H 2 O 0.09g, Na 2 MoO 4 2H 2 O 0.12g, H 3 BO 3 0.006g). First, add 3 g of sludge to the inorganic salt medium containing 10 mg / L sulfide anisole, and after acclimatization for 7 days at 37°C, transfer it into the inorganic salt medium containing 20 mg / L sulfide anisole at an inoculum size of 5% by volume, and then acclimate For one week, gradually increase the concentration...

Embodiment 2

[0055] The Brevibacillus Potsdam GIGAN1 screened by the present invention has better degradation ability to sulfide anisole:

[0056] Weigh K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O 0.01g, ZnSO 4 ·7H 2 O 0.2g, CoCl 2 ·6H 2 O 0.09g, Na 2 MoO 4 2H2O 0.12g, H 3 BO 3 0.0.06g, then dilute to 1L with deionized water, pack 100ml / bottle into 250ml shaker flasks, sterilize at 121°C for 30min, take it out, add thioanisole and thioanisole under sterile conditions The concentration of ether is 2mg / L, 4mg / L, 8mg / L, 16mg / L, 24mg / L in turn. Inoculate the GIGAN1 strain preserved on the slant (the slant medium is beef extract peptone medium) into the beef extract peptone medium for culture, and activate the bacteria in a shaker at 30°C and 220r / min for 15h, with a volume percentage of 5% The amount of inoculum was inserted into the inorganic salt medium containi...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a Brevibacillus borstelensis strain having a capability of degrading thioanisole and application thereof. The Brevibacillus borstelensis strain having a capability of degrading thioanisole is named as Brevibacillus borstelensis GIGAN1 and was collected at China Center for Type Culture Collection in Wuhan University in Wuhan, Hubei province, China on November 8, 2012; and the collection number is CCTCC NO:M 2012451. The capability of the strain, of degrading thioanisole in the environment, can be up to 96.2%; and the strain can be used for degrading thioanisole in the environment, thereby achieving the purpose of environment renovation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Brevibacillus potsdam strain capable of degrading sulfide anisole and an application thereof. Background technique [0002] Thioanisole (MPS), also known as thioanisole, is mostly used as a chemical raw material, and is often used in the synthesis of medical drugs such as antibiotics and anti-ulcers, as well as agricultural drugs such as insecticides and fungicides. Due to its low olfactory threshold, it is classified as a malodorous compound. The pollution of such substances affects the psychology and senses of the living and working people in the surrounding environment, causing people to feel unpleasant, irritable and disgusting, reducing work efficiency and quality of life; at the same time, it will also bring serious harm to health, such as Harm the respiratory system, digestive system, blood circulation system and nervous system, etc., and affect the b...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B01D53/84B01D53/48C02F3/34B09C1/10C12R1/01A62D101/28C02F101/34
CPCY02A50/20
Inventor 李桂英张正雍安太成
Owner GUANGZHOU INST OF GEOCHEMISTRY - CHINESE ACAD OF SCI
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