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Kit for Qualitative Detection of Vitellin Phosphoprotein in Cyprinidae and Its Application

A technology for qualitative detection of yolk lipoprotein, applied in the field of aquaculture, can solve laborious, uneconomical, time-consuming and other problems

Inactive Publication Date: 2014-10-22
OCEAN UNIV OF CHINA
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  • Description
  • Claims
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Problems solved by technology

This method is time-consuming, laborious, and very uneconomical

Method used

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  • Kit for Qualitative Detection of Vitellin Phosphoprotein in Cyprinidae and Its Application
  • Kit for Qualitative Detection of Vitellin Phosphoprotein in Cyprinidae and Its Application
  • Kit for Qualitative Detection of Vitellin Phosphoprotein in Cyprinidae and Its Application

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Embodiment Construction

[0016] A kit for the qualitative detection of vitellin phosphoprotein in Cyprinidae, comprising a box containing: 1) 2 PVDF membranes; 2) horseradish peroxidase-labeled goat anti-mouse secondary antibody 1 3) One each of blocking solution, washing solution and color developing solution, the washing solution is TBST; the blocking solution is TBST containing 5% skim milk powder; the color developing solution is containing 0.06% (m / V) 3' 10mM Tris-HCl of diaminobenzidine; it is characterized in that the box body is also equipped with: 4) 1 pure goldfish yolk lipophosphoprotein; 5) 1 mouse anti-goldfish yolk lipophosphoprotein polyclonal antibody.

[0017] The pure goldfish yolk lipophosphoprotein above is a 100 μg / ml goldfish yolk lipophosphoprotein solution.

[0018] 4) the pure product of goldfish yolk lipophosphoprotein in the above kit is prepared by the following method:

[0019] The ovary tissue of female goldfish in the late stage of yolk formation was taken, the connecti...

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Abstract

The invention discloses a kit for the qualitative detection of lipovitellin of cyprinid fish and the application thereof. The kit comprises goldfish lipovitellin products and mouse-anti goldfish lipovitellin polyclonal antibody. The method comprises the steps of firstly performing purifying to obtain goldfish lipovitellin, preparing mouse-anti goldfish lipovitellin polyclonal antiserum through immunized mice, and performing further purifying to obtain the mouse-anti goldfish lipovitellin polyclonal antibody. The kit can be used for identification and qualitative analysis of ovary of cyprinid fish and in vivo lipovitellin of larva fish, avoids traditional complex methods, such as sugar, phosphorus and fat dyeing and amino acid composition analysis, reduces financial resources, time and workload to a certain degree, and greatly improves the detection sensitivity on the basis that the work efficiency is improved, and the minimum detectability is about 100 ng / ml.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a kit for qualitatively detecting yolk lipophosphoprotein of carps and an application thereof. Background technique [0002] The development of fish is divided into larval stage, juvenile stage, juvenile stage, immature stage, adult stage and senescent stage. The division of each stage is directly related to the development of fish gonads. For female fish, the The main substance in the ovary is vitellin, which plays an important role in the whole life cycle of fish. First of all, vitellin is an important substance of fertilized eggs, providing nutrients and energy sources for fish embryo development and early growth of larvae. Secondly, during the vitellogenesis period, the egg cell absorbs vitellogenin from the blood, and after processing, it is stored in the oocyte in the form of yolk grains and oil droplets. The content of vitellogenin directly affects the survival of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/531G01N21/78
Inventor 汝少国王军王蔚田华
Owner OCEAN UNIV OF CHINA
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