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Novel temperature response micro-fluidic cell culturing chip and preparation method thereof

A temperature-responsive, microfluidic chip technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as no substantial breakthroughs, and achieve good application prospects, Ease of operation and effect of simplified cell engraftment process

Inactive Publication Date: 2013-04-24
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microfluidic chips are used to prepare microstructures and microchannels on its surface, relying on the laminar flow effect between multiple layers of liquid in the microchannel to drive sample microfluidics, and at the same time complete the implantation of various cells and the influence of in situ temperature changes. Cell growth technology has not yet had a substantial breakthrough in related application fields

Method used

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  • Novel temperature response micro-fluidic cell culturing chip and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0025] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip suitable for cell culture that contains six inlets, one main channel for cell culture, and six outlets. Add the cell mixture from the upper solution inlet to control the liquid difference height between the inlet and the outlet, thereby controlling the l...

Embodiment 2

[0027] The microstructure and microchannel graphics of the two-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructure and microchannel of the two-layer disc-shaped polymethyl methacrylate (PMMA) chip were processed and prepared by the numerical control CNC system, and the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the chip surface were wiped with ethanol . On the double-sided adhesive film, the required microstructures and microchannels were processed with a cutting machine. The two-layer chip is carefully aligned, glued, and pressure-sealed to make a microfluidic chip suitable for cell culture that contains six inlets, one main channel for cell culture, and six outlets. Add the cell mixture from the upper solution inlet to control the liquid difference height between the inlet and the outlet, thereby controlling the l...

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Abstract

The invention relates to a novel temperature response micro-fluidic cell culturing chip and a preparation method thereof. A microstructure and a micro-channel are arranged on the surface of the micro-fluidic chip; the surface of the micro-channel is decorated by bovine serum albumin molecule; under the driving of gravity generated by liquid level difference between a solution inlet and a solution outlet, cells are implanted in the micro-channel by using laminar flow phenomenon between liquid flows; finally, cell culture solutions with different temperatures are added from the solution inlet, so that the cells of the micro-channel are changed at different temperatures; therefore, the influence of temperature change on cell culture is studied and a basis is laid for studying cell biology; and the novel temperature response micro-fluidic cell culturing chip provided by the invention is mainly applied to the related field of cell biology, genetics, drug screening and the like. The micro-fluidic chip provided by the invention has the advantages as follows: the micro-fluidic chip is simple in operation, implantation and culture of the cells are finished, the consumption of reagents and samples is reduced, the cell implanting process is simplified, the influence of temperature change on cell culture is studied, the micro-fluidic chip is portable, economic, rapid, efficient and accurate, and a brand new cell culturing and analyzing technology is provided for studies on cell biology.

Description

technical field [0001] The invention relates to a novel temperature-responsive microfluidic cell layer culture chip and a preparation method thereof. The surface of the microfluidic chip has microstructures and microchannels, and the surface of the microchannel is modified with bovine serum albumin molecules. Driven by the gravity caused by the liquid difference between the outlets, the laminar flow phenomenon between the liquid flows is used to realize the implantation of cells in the microchannel, and finally the cell culture solutions of different temperatures are added from the solution inlet, and the cells in the microchannel are at different temperatures. It can produce changes under the environment, realize the study of the influence of temperature changes on cell culture, and lay the foundation for the study of cell biology. It is mainly used in related fields such as cell biology, genetics, and drug screening. Background technique [0002] In the late 1980s, in orde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 沙俊
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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