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Active mold inhibitor, as well as preparation method and application thereof

A mold and preparation technology, applied in the field of applied microorganisms, can solve the problems that there are not many reports on the research of active mold preparations, and achieve the effects of short cycle, convenient storage and convenient quantification

Active Publication Date: 2015-07-15
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the direct-throwing live bacteria preparation has been widely used in fields such as agriculture, food, medicine, and sanitation. The patent of publication number CN1602764 discloses a kind of direct-throwing compound bacterial powder product and production method for producing kimchi, and the patent of publication number CN101486987 The patent discloses a method for preparing freeze-dried bacteria powder of bifidobacteria, but these are studies on live bacterial preparations, and there are not many reports on active mold preparations

Method used

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  • Active mold inhibitor, as well as preparation method and application thereof
  • Active mold inhibitor, as well as preparation method and application thereof
  • Active mold inhibitor, as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: Active mold preparation is prepared by the following method:

[0026] (1) Inoculation and fermentation conditions: 1% inoculation amount of spore liquid, 200r / min vibration, 26°C for 40 hours, inoculated in 10L fermenter according to 3%-5% inoculum amount, fermentation medium components and first-grade seeds The composition of the culture medium is the same. At a temperature of 29°C and a ventilation ratio of 1:1, after 48 hours of cultivation, the fermentation broth is vacuum-filtered through a 200-mesh nylon filter cloth frame to obtain wet cells.

[0027] (2) Dry protection agent treatment: The dry protection agent is compounded with soluble starch, soybean powder, and glycerin. The compound ratio is 1:0.1:0.02, 121 ° C, 20 minutes of sterilization treatment, and the water is 121 ° C. Sterilize for 20 minutes and wait for use;

[0028] (3) Drying process: the above-mentioned sterilized water and drying aid protection agent are mixed according to the ma...

Embodiment 2

[0030] Embodiment 2: Active mold preparation is prepared by the following method:

[0031] (1) Inoculation and fermentation conditions: 5% inoculation amount of spore liquid, shaking at 220r / min, cultured at 29°C for 36 hours, then inoculated in a 10L fermenter according to 3% inoculum amount, the composition of the fermentation medium and the primary seed medium The ingredients are the same, at a temperature of 29°C and a ventilation ratio of 1:0.8, after 48 hours of cultivation, the fermentation broth is vacuum-filtered through a 300-mesh nylon filter cloth frame to obtain wet cells;

[0032] (2) Treatment of dry protection agent: The dry protection agent is compounded with soluble starch, soybean powder and glycerin, the compound ratio is 1:0.1:0.02, sterilized at 121°C for 20 minutes for use, water is passed through 121°C, Sterilize for 20 minutes and wait for use;

[0033] (3) Drying process: the above-mentioned sterilized water and drying aid protection agent are mixed ...

Embodiment 3

[0036] Embodiment 3: Active mold preparation is prepared by the following method:

[0037] (1) Inoculation and fermentation conditions: 10% inoculation amount of spore liquid, 200r / min shaking, 36-40 hours of cultivation at 29°C, inoculation in 10L fermenter according to 3%-5% inoculum amount, fermentation medium components and a The ingredients of the seed culture medium are the same. At a temperature of 29°C and a ventilation ratio of 1:1.2, after 48 hours of cultivation, the fermentation broth is vacuum-filtered through a 300-mesh nylon filter cloth frame to obtain wet cells;

[0038] (2) Drying aid and protection agent treatment: the drying aid and protection agent is compounded with soluble starch, soybean powder, and glycerin, sterilized at 121°C for 20 minutes for use, and water is sterilized for 20 minutes at 121°C for use;

[0039] (3) Drying process: the above-mentioned sterilized water and drying aid protection agent are mixed according to the mass ratio of 1:1 to f...

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Abstract

The invention discloses an active mold inhibitor, as well as a preparation method and an application thereof, and specifically provides a conveniently and easily operated bacterial agent for preparing a mycelium blank by a white blank in the process of manufacturing a fermented bean curd. According to the active mold inhibitor, thalli are obtained by performing plate-frame filtration on actinomucor elegans that is cultivated by a fermentation tank in a liquid state; the thalli are compounded with a corn starch or a soluble starch, soybean meal and glycerol according to a certain ratio to form a drying-assisting protective agent; and massive mycelia can be rapidly multiplied and grown on the surface of the bean curd, thereby covering the white blank of the bean curd and forming the mycelium blank that is dense in texture. According to the invention, the bacterial agent is used for manufacturing a fermented bean curd, is short in growth cycle, can become a dominant flora within only 36-48h, and achieves the maximum mycelium length and the dense coverage so as to avoid the invasion of sundry bacteria; the bacterial agent is simple in manufacturing method, easy to extract at any time, and high in activity; and the content of flora in per unit of the bacteria agent is maximum.

Description

technical field [0001] The invention relates to the preparation of microbial bacterial agents, in particular to an active mold preparation and its preparation method and application, belonging to the field of applied microorganisms. Background technique [0002] Molds are widely used in the food processing industry. Many fermented foods and food raw materials are produced and processed with the participation of molds, such as fermented bean curd, tempeh, soy sauce, citric acid, etc. Recent studies have proved that mucormyces can be used in Production of new foods such as cheese. [0003] Due to the current production of fermented bean curd, although most of the traditional natural fermentation process has been changed to the pure strain fermentation process, since the production still adopts open natural environment culture, the external microorganisms will inevitably invade, and the ingredients also contain microorganisms. Therefore, it is very important to control the gro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/04A23C20/02C12R1/645A23L11/45A23L11/50
Inventor 汪建明马晓蕾乔长晟
Owner 天津北洋百川生物技术有限公司
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