Composition based on main components of Pu'er tea and application thereof

Abstract

The invention discloses composition based on main components of Pu'er tea and the application thereof. The composition comprises tea brown pigment, tea polysaccharide, tea polyphenol, caffeine, gamma-aminobutyric acid and gallic acid. A wild type mouse embryo fibroblast is utilized to test the killing effect of DNA toxicity chemical substance to normal cells and the effect that the composition provided by the invention protects the normal cells from DNA damage and prevents apoptosis; the result shows that the composition provided by the invention can effectively prevent the normal cell death caused by DNA toxicity chemical substance; and further research shows that the composition provided by the invention does not protect tumor cell death caused by the DNA toxicity chemical compound, conversely, the composition provided by the invention can promote the killing effect of the DNA toxicity chemical compound to the tumor cells. Therefore, the composition can be used for protecting the normal cells during the chemotherapy of tumor patients and alleviating toxic side effects caused by tumor chemotherapy, and meanwhile sensibilizes chemotherapy drug, enhances chemotherapy effect, and improves survival quality of chemotherapy patients.

Description

technical field

[0001] The invention relates to a composition based on the main component of Pu'er tea and its application, more specifically, the invention relates to the application of the composition in the prevention of biological hazards caused by DNA toxicity chemicals. Background technique

[0002] DNA toxicity or genotoxic chemicals refer to a large class of chemicals that can cause DNA damage, leading to gene mutation, cell death or tumorigenesis. These include directly binding to DNA, chemically modifying DNA, causing DNA damage; or acting on DNA-binding related proteins, causing damage to DNA, etc. Most of the teratogenic, carcinogenic and carcinogenic compounds belong to this category, and this kind of compounds are often encountered in people's daily life, such as automobile exhaust, cigarette smoke, kitchen fume and so on.

[0003] Due to the abnormally active proliferative ability of tumor cells, intracellular DNA replication is very active, which has also be...

Examples

Embodiment 1

[0033] Example 1: The composition based on the main components of Pu'er tea in the present invention, its composition and mass percentage are 50% theabrownin, 30% tea polysaccharides, 11% tea polyphenols, 5% caffeine, 2% γ-amino Butyric acid and 2% gallic acid.

[0034] This example uses wild-type mouse embryo fibroblast (mouse embryo fibroblast, MEF) to test the killing effect of DNA toxic compounds on normal cells, and at the same time test the protective effect of the composition of the present invention on normal cells, thereby revealing the composition Efficacy in preventing biohazards from DNA toxic compounds;

[0035] In this example, the Annexin V-FITC cell apoptosis detection kit was used to detect cell apoptosis and the protective effect of the composition. The specific operation was as follows: the inoculation concentration was 1×10 5 mouse embryonic fibroblasts onto glass slides in six-well cell culture plates, using DMEM + 10% fetal bovine serum medium, at 37°C, ...

Embodiment 2

[0047] Example 2: The composition based on the main components of Pu'er tea in the present invention, its composition and mass percentage are 5% theabrownin, 40% tea polysaccharides, 30% tea polyphenols, 23% caffeine, 1% γ-amino Butyric acid and 1% gallic acid.

[0048] This example utilizes tumor cell p53 - / - +S+Ras tests the killing effect of DNA toxic compounds on tumor cells, and at the same time tests the sensitization effect of the composition of this example on this killing, thereby revealing the efficacy of this composition in clinical adjuvant chemotherapy. The specific operation is as follows: inoculation The concentration is 1×10 5 tumor cell p53 - / - +S+Ras onto glass slides in six-well cell culture plates using DMEM+10% fetal bovine serum medium at 37°C, 5% CO 2 , 3% O 2 Cultivate in an incubator until the cells adhere to the wall; divide the cells into a test group and a control group, add the composition of this embodiment to the test group for co-cultivation...

Embodiment 3

[0059] Example 3: The composition based on the main components of Pu'er tea in the present invention, its composition and mass percentage are 14.8% theabrownin, 5% tea polysaccharides, 50% tea polyphenols, 30% caffeine, 0.1% γ-amino Butyric acid and 0.1% gallic acid.

[0060] In this example, cyclophosphamide is used as a DNA toxic compound, and tumor cell p53 is utilized - / - +S+Ras tests the killing effect of DNA toxic compounds on tumor cells, and at the same time tests the sensitization effect of the composition of this example on this killing, thereby revealing the efficacy of this composition in clinical adjuvant chemotherapy. The specific operation is as follows: inoculation 5×10 3 tumor cell p53 - / - +S+Ras into a 96-well cell culture plate, use DMEM+10%FBS medium, divide the test samples into a test group and a control group, add the composition of this embodiment to the test group for co-cultivation, and the control group does not add this Example Composition Co-cul...

technical field

[0001] The invention relates to a composition based on the main component of Pu'er tea and its application, more specifically, the invention relates to the application of the composition in the prevention of biological hazards caused by DNA toxicity chemicals. Background technique

[0002] DNA toxicity or genotoxic chemicals refer to a large class of chemicals that can cause DNA damage, leading to gene mutation, cell death or tumorigenesis. These include directly binding to DNA, chemically modifying DNA, causing DNA damage; or acting on DNA-binding related proteins, causing damage to DNA, etc. Most of the teratogenic, carcinogenic and carcinogenic compounds belong to this category, and this kind of compounds are often encountered in people's daily life, such as automobile exhaust, cigarette smoke, kitchen fume and so on.

[0003] Due to the abnormally active proliferative ability of tumor cells, intracellular DNA replication is very active, which has also be...

Examples

Embodiment 1

[0033] Example 1: The composition based on the main components of Pu'er tea in the present invention, its composition and mass percentage are 50% theabrownin, 30% tea polysaccharides, 11% tea polyphenols, 5% caffeine, 2% γ-amino Butyric acid and 2% gallic acid.

[0034] This example uses wild-type mouse embryo fibroblast (mouse embryo fibroblast, MEF) to test the killing effect of DNA toxic compounds on normal cells, and at the same time test the protective effect of the composition of the present invention on normal cells, thereby revealing the composition Efficacy in preventing biohazards from DNA toxic compounds;

[0035] In this example, the Annexin V-FITC cell apoptosis detection kit was used to detect cell apoptosis and the protective effect of the composition. The specific operation was as follows: the inoculation concentration was 1×10 5 mouse embryonic fibroblasts onto glass slides in six-well cell culture plates, using DMEM + 10% fetal bovine serum medium, at 37°C, ...

Embodiment 2

[0047] Example 2: The composition based on the main components of Pu'er tea in the present invention, its composition and mass percentage are 5% theabrownin, 40% tea polysaccharides, 30% tea polyphenols, 23% caffeine, 1% γ-amino Butyric acid and 1% gallic acid.

[0048] This example utilizes tumor cell p53 - / - +S+Ras tests the killing effect of DNA toxic compounds on tumor cells, and at the same time tests the sensitization effect of the composition of this example on this killing, thereby revealing the efficacy of this composition in clinical adjuvant chemotherapy. The specific operation is as follows: inoculation The concentration is 1×10 5 tumor cell p53 - / - +S+Ras onto glass slides in six-well cell culture plates using DMEM+10% fetal bovine serum medium at 37°C, 5% CO 2 , 3% O 2 Cultivate in an incubator until the cells adhere to the wall; divide the cells into a test group and a control group, add the composition of this embodiment to the test group for co-cultivation...

Embodiment 3

[0059] Example 3: The composition based on the main components of Pu'er tea in the present invention, its composition and mass percentage are 14.8% theabrownin, 5% tea polysaccharides, 50% tea polyphenols, 30% caffeine, 0.1% γ-amino Butyric acid and 0.1% gallic acid.

[0060] In this example, cyclophosphamide is used as a DNA toxic compound, and tumor cell p53 is utilized - / - +S+Ras tests the killing effect of DNA toxic compounds on tumor cells, and at the same time tests the sensitization effect of the composition of this example on this killing, thereby revealing the efficacy of this composition in clinical adjuvant chemotherapy. The specific operation is as follows: inoculation 5×10 3 tumor cell p53 - / - +S+Ras into a 96-well cell culture plate, use DMEM+10%FBS medium, divide the test samples into a test group and a control group, add the composition of this embodiment to the test group for co-cultivation, and the control group does not add this Example Composition Co-cul...

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