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Preparation method and device for novel labeling technique of cervix cancer cells
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A marking technology, the technology of cervix, applied in the field of preparation of marking technology
Active Publication Date: 2013-05-01
GUANGZHOU HONGQI OPTICAL INSTR TECH
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[0004] However, pathological diagnosis using ThinPap liquid-based cytology test not only re
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no. 1 example
[0051] see figure 1 , the first embodiment of the preparation method of the new cervical cancer cell labeling technology in the embodiment of the present invention includes:
[0052] 101. Preparation of colloidal gold;
[0053] The main component of colloidal gold is chloroauric acid. The preparation method can be trisodium citrate reduction method. The commonly used reducing agents are trisodium citrate, tannic acid, ascorbic acid, white phosphorus and sodium borohydride.
[0054] 102. Using colloidal gold and antibodies against epithelial cell adhesion molecules to prepare immunogold;
[0055] After the colloidal gold is prepared, immunogold can be prepared by using the colloidal gold and the antibody against epithelial cell adhesion molecules.
[0056] 103. Using immunogold to label the epithelial cell adhesion molecules in the pre-collected samples;
[0057] After the immunogold is prepared, the immunogold can be used to label the epithelial cell adhesion molecules in t...
no. 2 example
[0061] The first embodiment of the preparation method of the new cervical cancer cell marking technology of the present invention is briefly introduced above, and the second embodiment of the preparation method of the new cervical cancer cell marking technology of the present invention is described in detail below, please refer to figure 2 , the second embodiment of the preparation method of the new cervical cancer cell labeling technology in the embodiment of the present invention includes:
[0062] 201, preparation of colloidal gold;
[0063] The main component of colloidal gold is chloroauric acid. The preparation method can be trisodium citrate reduction method. The commonly used reducing agents are trisodium citrate, tannic acid, ascorbic acid, white phosphorus and sodium borohydride. The principle and specific operation method are as follows:
[0064] 2HAuCl 4 +3C 6 h 8 o 7 =2Au+3C 5 h 6 o 5 +8HCl+3CO 2
[0065] The specific method of preparing colloidal gold...
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Abstract
The embodiment of the invention discloses a preparation method and device for a novel labeling technique of cervix cancer cells; and the method and the device can simply, quickly and accurately diagnose the cervix cancer cells. The method provided by the embodiment comprises the following steps: preparing colloidal gold; using the colloidal gold and antibodies for resisting epithelial cell adhesion molecules to prepare immunogold; and using the immunogold to label the epithelial cell adhesion molecules in pre-collected samples, and tabletting the samples. As colloidal gold particles are visible under a general optical microscope, when the epithelial cell adhesion molecules are labeled by using the colloidal gold and the immunogold which is prepared by the antibodies for resisting the epithelial cell adhesion molecules, the property of the epithelial cell adhesion molecules can be determined through direct observation; and as the expression level relevancy of the cervix cancer cells with the epithelial cell adhesion molecules on the surfaces of the cells is higher, the cervix cancer cells can be simply, quickly and accurately diagnosed through the preparation method and device in the embodiment of the invention.
Description
technical field [0001] The embodiments of the present invention relate to the field of biomedical technology, and in particular to a preparation method and device for a new cervical cancer cell marking technology. Background technique [0002] Cervical cancer is one of the most common malignant tumors, and its incidence rate ranks second among female tumors. About 200,000 women die from this disease every year in the world, and early diagnosis and treatment of cervical precancerous lesions can reduce its incidence. [0003] At present, a relatively advanced cervical cancer detection technology in the world is Thinprep Cytologic Test (TCT, Thinprep Cytologic Test). TCT inspection uses liquid-based thin-layer cytology detection system to detect cervical cells and conduct cytological classification diagnosis. Compared with the traditional cervical smear and Pap smear, it significantly improves the satisfaction of the specimen and the detection rate of abnormal cervical cells. ...
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