Wax cell chip inoculation method

Abstract

The invention provides a wax cell chip inoculation method which is characterized by comprises the following specific steps of: wrapping cells by using casings which are subjected to fixation by formalin, treating and dipping wax so as to obtain wax-dipped cell specimens; heating to the wax-dipped cell specimens to be in a molten state, opening the casings, scrapping the cells and liquid wax into an EP (Epoxy Resin) tube, uniformly mixing, molding into the cell chips in desired shapes, putting a wax mold preparation frame on a wax mold plate, pouring the wax into the wax mold preparation frame, polymerizing, taking gout and detaching down the wax mold plate; putting an obtained wax mold into an incubator together with the wax mold frame, slightly softening the wax mold and taking the softened the wax mold out, drilling holes within an inoculation range of the wax mold, and inoculating the cell chips into corresponding holes of the wax mold; putting the wax mold into a constant temperature incubator to be polymerized; and putting the wax mold and the wax mold frame into a refrigerator, taking the wax mold and the wax mold frame out, separating the wax mold from the wax mold preparation frame by laterally knocking the wax mold preparation frame, and slicing the obtained wax mold so as to obtain the wax cell chips. The wax cell chip inoculation method has the advantages of low cost, convenience, rapidness, high yield and diversity in molding shapes.

Description

technical field [0001] The invention relates to a paraffin cell chip seeding method, which is used for preparing paraffin cell chips. Background technique [0002] Cell chip technology is the development and extension of gene cell chip technology. Like tissue cell chip, protein cell chip, and antibody cell chip, it belongs to a special biological cell chip technology. The cell chip technology can arrange dozens or even thousands of clinical cell specimens from different individuals on a glass slide in a pre-designed sequence for analysis and research. It is a high-throughput, multi-sample analysis tool. Cell chip technology enables researchers to analyze hundreds or even thousands of tissue samples at the same time, and conduct research on one or more specific genes or their related expression products. This technology can not only be combined with traditional pathological techniques, histochemical and immunohistochemical techniques, but also with DNA, RNA, protein, antibod...

AI Technical Summary

Problems solved by technology

[0004] However, the preparation of cell chips according to the above-mentioned method has the following disadvantages: first, the production cost of existing cell chips is very high

In addition to special equipment (hundreds of thousands of yuan), the cost of making a cell chip is more than 10,000 yuan, and the unit price of each slice is 50-100 yuan; second, sampling can only be done on the existing paraffin cell block, The source of tissue is limited; third, the cell chip prepared according to the existing equipment and methods only has about 50 slices, because the sample is made by punching holes on the original wax block, and the thickness of the cell sample is limited; fourth, although the high-density cell chip The throughput is large, but it is difficult to provide sufficient information due to the small size of each tissue; fifth, the cells are easy to diffuse during the aggregation process, resulting in mutual contamination between different cases; sixth, only in large research units, there is funding and conditions to carry out Research on cell chips is difficult to promote

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