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Chitosan nano particle, biological pesticide preparation for controlling tobacco caterpillars and preparation method thereof

A technology of chitosan nanoparticles and Spodoptera litura, which is applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of unrealized standardization level, nanoparticle size distribution, and drug loading rate. Problems such as unstable indicators and small molecular weight have achieved breakthroughs in the scale of laboratory operations, high pest control effects, and standardized production steps.

Active Publication Date: 2013-05-22
GUANGZHOU RIBOBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above reports have successfully prepared pesticide chitosan nanoparticle preparations, their research and development are still in the exploratory stage, and the physical and chemical indicators such as the size, particle size distribution, and drug loading rate of the prepared nanoparticles are unstable, and the standardization level has not been achieved. It is cumbersome and difficult to achieve large-scale production, so it has been limited to laboratory research so far, and has not been transformed into a product preparation circulation market
In addition, the active ingredients of the pesticide chitosan preparations successfully reported so far are mostly existing chemical pesticides with small molecular weight and relatively simple technical operation.

Method used

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  • Chitosan nano particle, biological pesticide preparation for controlling tobacco caterpillars and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling tobacco caterpillars and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling tobacco caterpillars and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation with the double-stranded ribonucleic acid sequence of Spodoptera litura acetylcholinesterase gene as the active ingredient.

[0046] (1) Design and synthesis of original drug components targeting spodoptera litura acetylcholinesterase gene double-stranded ribonucleic acid

[0047] GenBank: FJ959384.1

[0048] Si-Ace1_001: target gene CAGGA ACTGC TACTC TA

[0049] Sense strand (5'-3'): 5'CAGGA ACUGC UACUC UA dTdT3' SEQ ID NO.1

[0050] Antisense strand (3'-5'): 3'dTdT GUCCU UGACG AUGAG AU5' SEQ ID NO.2

[0051] Si-Ace1_002: TGTCG GTTTC ATTGC AT

[0052] Sense strand (5'-3'): 5'UGUCG GUUUC AUUGC AU dTdT3' SEQ ID NO.3

[0053] Antisense strand (3'-5'): 3'dTdT ACAGC CAAAG UAACG UA5' SEQ ID NO.4

[0054] Si-Ace1_003: GGCGC ATCGT TATGC AG

[0055] Sense strand (5'-3'): 5'GGCGC AUCGU UAUGC AG dTdT3' SEQ ID NO.5

[0056] Antisense strand (3'-5'): 3'd...

Embodiment 2

[0077] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation targeting the double-stranded ribonucleic acid sequence of Spodoptera litura ecdysone receptor gene as the active ingredient.

[0078] (1) Design and synthesis of active ingredients for dsRNA of Spodoptera litura ecdysone receptor gene

[0079] GenBank:EU180021.1

[0080] Si-EcR_001: GTTGC TCGGA TTGTG TTGT

[0081] Sense strand (5'-3'): 5'GUUGC UCGGA UUGUG UUGU dTdT3' SEQ ID NO.7

[0082] Antisense strand (3'-5'): 3'dTdT CAACG AGCCU AACAC AACA5' SEQ ID NO.8

[0083] Si-EcR_002: GAGTG GTGCA TGTCC ATAG

[0084] Sense strand (5'-3'): 5'GAGUG GUGCA UGUCC AUAG dTdT3' SEQ ID NO.9

[0085] Antisense strand (3'-5'): 3'dTdT CUCAC CACGA ACAGG UAUG5' SEQ ID NO.10

[0086] Si-EcR_003: TCGCA AAGAT CTCAC AATC

[0087]Sense strand (5'-3'): 5'UCGCA AAGAU CUCAC AAUC dTdT3' SEQ ID NO.11

[0088] Antisense strand (3'-5'): 3'dTdT AGCGU UUCUA GAGUG UUAG5' SEQ ID ...

Embodiment 3

[0110] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation targeting the double-stranded ribonucleic acid sequence of the γ-aminobutyric acid receptor α subunit gene of Spodoptera litura as the active ingredient.

[0111] (1) Design and synthesis of double-stranded ribonucleic acid technical drug targeting Spodoptera litura γ-aminobutyric acid receptor α subunit gene

[0112] GenBank: JN792582.1

[0113] Si-GABA_001: CTGAA GTGAA AATGG AT

[0114] Sense strand (5'-3'): 5'CUGAA GUGAA AAUGG AU dTdT3' SEQ ID NO.13

[0115] Antisense strand (3'-5'): 3'dTdT GACUU CACUU UUACC UA5' SEQ ID NO.14

[0116] Si-GABA_002: TATGC AATAT TGAAA TC

[0117] Sense strand (5'-3'): 5'UAUGC AAUAU UGAAA UC dTdT3' SEQ ID NO.15

[0118] Antisense strand (3'-5'): 3'dTdT AUACG UUAUA ACUUU AG5' SEQ ID NO.16

[0119] Si-GABA_003: GCGAC ACCTG CTAGG GT

[0120] Sense strand (5'-3'): 5'GCGAC ACCUG CUAGG GU dTdT3' SEQ ID NO.17

[0121...

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Abstract

The invention discloses a chitosan nano particle which comprises the following raw materials in percentage by weight: 0.5-5% of chitosan, 1-10% of cosolvent, 0-5% of antifreezing agent, 0.1-1% of surfactant, 0.1-0.5% of emulsifier, 40-60% of buffer solution and the balance of water. The invention also provides a biological pesticide preparation for controlling tobacco caterpillars and a preparation method thereof. The biological pesticide preparation mainly comprises the following components in percentage by weight: (A) 0.05-15% of active component: a siRNA (small interfering ribonucleic acid) is designed and synthesized in accordance with a tobacco caterpillar high-efficiency killing target gene and is a 15-50nt double-chain RNA; (B) 19.95-55% of solvent; and (C) 30-80% of chitosan nano particle. The chitosan nano particle ensures that the stability and the control effect of the biological pesticide preparation are better.

Description

technical field [0001] The invention belongs to the technical field of agricultural insecticides, and specifically relates to a biological pesticide preparation and a preparation method thereof, in particular to a chitosan nanoparticle, a biological pesticide preparation containing double-stranded ribonucleic acid for controlling Spodoptera litura and a preparation method thereof. technical background [0002] Spodoptera litura belongs to Lepidoptera, Noctuidae insects, which can harm many vegetables and crops such as Brassicaceae, Solanaceae, Leguminosae, Cucurbitaceae, onion and garlic, etc. It is also common on weeds and wild vegetables, and is a kind of food It is a very complex insect with more than 200 kinds of host plants. Since the 1990s, the middle and lower reaches of the Yangtze River have been plagued by outbreaks almost year after year, and have become one of the most important pests of cruciferous vegetables and flowers and plants in urban green spaces. Becaus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/10A01N43/90A01P7/04
Inventor 张必良
Owner GUANGZHOU RIBOBIO
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