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Method for removing ginkgolic acid and heavy metals in ginkgo biloba extract by using nanometer material

A nanomaterial, ginkgo biloba technology, applied in the fields of medical raw materials derived from Ginkgo biloba, chemical/physical/physical-chemical processes of applied energy, and drug combinations, etc. question

Active Publication Date: 2015-06-17
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solvent extraction is very prone to emulsification, which leads to prolonged production time and incomplete extraction. There will be solvent residues in the product, and it is not friendly to operators and the environment.
The patent document "Removal process of ginkgolic acid in ginkgo leaf extract" (CN101194918A) adopts double-column stacking method to remove ginkgolic acid, but the fabrication is relatively complicated, and the pretreatment and activation of the double-column will produce a certain amount of industrial wastewater
[0004] The excessive content of ginkgolic acid and heavy metals in Ginkgo biloba extract is the bottleneck restricting the export of Ginkgo biloba extract in my country, and also affects the safety of domestic pharmaceuticals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 500g of Ginkgo biloba leaves, add 10, 8 times the amount of 60% ethanol, reflux and extract for 2 hours respectively, filter the medicinal solution, combine, recover the ethanol under reduced pressure until the medicinal solution does not contain alcohol, then add water to 1500ml, let stand, filter, add the filtrate Wash the treated DA201 resin column with pure water. When the effluent is clear, elute with 2000mL80% ethanol, collect the ethanol eluate, add 0.2g ferric oxide nanomaterials, ultrasonically adsorb for 2 hours, and put the magnet in The ferric oxide nanometer material is adsorbed in the medicinal liquid, the medicinal liquid is concentrated under reduced pressure to recover ethanol, and the extract is vacuum-dried. The iron ferric oxide nanomaterials were collected, soaked in 0.1% hydrochloric acid for 4 hours, magnetically separated, rinsed with deionized water several times, and dried in vacuum for later use. After drying, the Ginkgo biloba extract wa...

Embodiment 2

[0025] Take 500g of Ginkgo biloba leaves, add 8 and 6 times the amount of 70% ethanol, reflux and extract for 1.5h respectively, filter the medicinal solution, combine, recover ethanol under reduced pressure until the medicinal solution does not contain alcohol, then add water to 1500ml, let stand, filter, and filtrate Add to the treated D101 resin column, clean the column with pure water, when the effluent is clear, elute with 2500mL80% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure until the solid content of the liquid reaches 1.08-1.10, Stop concentrating, add 0.5g Fe3O4 nanometer material to the liquid medicine, ultrasonically adsorb for 5 hours, put the electromagnet into the liquid medicine to absorb the Fe3O4 nanometer material, concentrate the medicine liquid under reduced pressure, and when the solid content reaches 1.25-1.30, Spray dry. The iron ferric oxide nanomaterials were collected, soaked in 1% hydrochloric acid for 2 hours, magneti...

Embodiment 3

[0027] Take 500g of Ginkgo biloba leaves, add 10, 5 times the amount of 70% ethanol, reflux and extract for 3 hours respectively, filter the medicinal solution, combine them, recover the ethanol under reduced pressure until the medicinal solution does not contain alcohol, then add water to 1500ml, let stand, filter, add the filtrate Wash the treated AB-8 resin column with purified water. When the effluent is clear, elute with 2000mL60% ethanol, collect the ethanol eluate, add 1.0g ferric oxide nanomaterial, ultrasonic adsorption for 8h, magnet Putting into the liquid medicine to absorb ferric iron tetroxide nanomaterials, the liquid medicine is concentrated under reduced pressure, and when the solid content reaches 1.25-1.30, it is spray-dried. The iron ferric oxide nanomaterials were collected, soaked in 2% hydrochloric acid for 24 hours, magnetically separated, rinsed with deionized water several times, and dried in vacuum for later use. After drying, the Ginkgo biloba extra...

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PUM

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Abstract

The invention discloses a method for removing ginkgolic acid and heavy metals in a ginkgo biloba extract by using a nanometer material. The method is characterized in that the ginkgo biloba extract is obtained by extraction, column chromatography, adsorption of the nanometer material, separation of the nanometer material, concentration and drying of separation liquid; a ferroferric oxide nanometer material is used as the nanometer material; and a magnetic separation process is used as a nanometer material separation process. As the improvement, the method further can comprise a step of recovering the nanometer material. According to the method provided by the invention, the ginkgolic acid, urushiol, isourushiol and the heavy metals are adsorbed by a Fe3O4 nanometer material; solid-liquid separation is carried out by using the magnetic separation process; and a finished product can be obtained by concentrating and drying the medicine liquid. The method provided by the invention has the double effects of environmental friendliness and simultaneous removal of the ginkgolic acid and the heavy metals.

Description

technical field [0001] The invention belongs to the technical field of natural product preparation, and in particular relates to a method for removing ginkgolic acid and heavy metals in ginkgo biloba extract by using nanometer material adsorption. Background technique [0002] Ginkgo biloba is the dried leaf of Ginkgo biloba (Ginkgo biloba L.), sweet, bitter, astringent, flat, homecoming, lung meridian, has the effects of astringing the lungs, relieving asthma, promoting blood circulation, removing blood stasis, and relieving pain. It is used for coronary heart disease , angina pectoris and hyperlipidemia. Ginkgo biloba extract is an extract processed from Ginkgo biloba leaves, which promotes blood circulation, removes blood stasis and dredges collaterals. It is used to treat chest pain, stroke, hemiplegia, tongue congestion caused by blood stasis, stable angina pectoris, and cerebral infarction. See above syndrome. Ginkgo biloba and ginkgo leaf extract are used as raw mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/16A61P9/10B01D15/08B01J19/10
Inventor 敖明章余龙江石振拓陶铸唐本兴石华杰
Owner HUAZHONG UNIV OF SCI & TECH