A novel screening method for anticancer drugs
A screening method and anti-cancer drug technology, applied in the field of molecular biology, can solve the problems of not being able to understand the mechanism of anti-cancer action, being easily affected by human error, consuming time and funds, etc., and achieving easy promotion, simple method, and few reagents Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0035] A novel screening method for anticancer drugs, the key of which is to include the following steps:
[0036] (1) Preparation of TTLL12 overexpression cell line
[0037] 1) PCR amplification of TTLL12 gene fragment
[0038] Self-designed primers, sense strand 5'-CGGGATCCCCGATGGACTACCACGAGGA-3', antisense strand 5'-CGGGATCCTAGCTACAGGACGCCCCCG-3', amplified the TTLL12 gene fragment using TTLL12 cDNA as a template. The size of the target gene fragment is 2818bp, the composition of PCR reaction solution: Ex Taq enzyme (5u / μL) 0.15μL, 10×PCR buffer 2.5μL, MgCL 2 (25mmol / L) 2μL, dNTP (each 2.5mmoL / L) 2μL, upstream primer (10mol / L) 1.0μL downstream primer (10mol / L) 1.0μL, template DNA 4.5μL, add sterilized pure water to 25μL. PCR cycle parameters: 94°C for 5min, 94°C for 50s, 55°C for 30s, 72°C for 30s, 35cycle, 72°C for 8min. Identification of PCR products: Separation and identification on 1% agarose gel electrophoresis. see results figure 2 .
[0039] 2) Insert the TTLL...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com