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Electrogenerated chemiluminescence sensor with high sensitivity in cancer cell detection and fabrication method of sensor

A sensitive detection and electrochemistry technology, applied in chemiluminescence/bioluminescence, scientific instruments, analysis by chemical reaction of materials, etc., can solve the problems of cumbersome operation, long analysis and detection time, etc. Strong and effective

Inactive Publication Date: 2015-04-01
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods have disadvantages such as relying on expensive analytical instruments, or long analysis and detection time, cumbersome operations, etc.

Method used

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  • Electrogenerated chemiluminescence sensor with high sensitivity in cancer cell detection and fabrication method of sensor
  • Electrogenerated chemiluminescence sensor with high sensitivity in cancer cell detection and fabrication method of sensor

Examples

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Effect test

Embodiment 1

[0021] Fabrication method of electrochemiluminescence sensor for highly sensitive detection of HepG2 liver cancer cells based on nanocomposite materials:

[0022] (1) Synthesis of nano-titanium dioxide material: first dissolve 0.25g of titanium sulfate in 90mL of distilled water, under magnetic stirring, slowly add 10mL of ethylenediaminetetraacetic acid with a concentration of 0.025M into the titanium sulfate solution until a white precipitate appears Afterwards, continue to react for 2 hours, centrifuge, wash, and dry at 60 degrees Celsius for 6 hours, and set aside. The above reactions except the last step were carried out at room temperature of 25°C.

[0023] (2) Synthesis of composite nanomaterial ionic liquid: ultrasonically clean titanium dioxide nanoparticles for 5 minutes, and disperse them in ultrapure water with a concentration of 5 mg / mL, and set aside. Add 1 mL of 0.2 mol / mL sodium sulfide solution and 1 mL of 0.2 mol / mL cadmium chloride solution successively to ...

Embodiment 2

[0030] Fabrication method of electrochemiluminescence sensor for highly sensitive detection of intestinal cancer cells based on nanocomposite materials

[0031] (1) Synthesis of nano-titanium dioxide material: In a 100mL three-necked flask, dissolve 0.35g of titanium sulfate in 100mL of distilled water, under magnetic stirring, slowly add 20mL of EDTA with a concentration of 0.05M into the three-necked flask with white After precipitation appeared, continue to react at room temperature for 2.5 hours, centrifuge, wash, and dry at 60° C. for 6.5 hours for later use. The above reactions were carried out at room temperature (25° C.) except for the last step.

[0032] (2) Synthesis of composite nanomaterial ionic liquid: ultrasonically clean titanium dioxide nanoparticles for 5 minutes, and disperse them in ultrapure water to reach a concentration of 5 mg / mL, and set aside; add them dropwise in 1 mL of dispersed nano titanium dioxide materials 1.5mL sodium sulfide solution with a ...

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Abstract

The invention relates to an electrogenerated chemiluminescence sensor with high sensitivity in cancer cell detection and a fabrication method of the sensor. The fabrication method comprises the following steps of (1) nanometer titania material synthesis: titanous sulfate reacts with ethylene diamine tetraacetic acid under magnetic stirring to produce nanometer titania; (2) composite nano material synthsis: titania nano particles sequentially reacts with a sodium sulphide solution and a cadmium chloride solution to be dissolved in ionic liquid; (3) gold nano particle synthesis: a sodium citrate solution reacts with a chloroauric acid solution to obtain gold nano particles; (4) assembling of the sensor: the gold nano particles are dropped into the surface of an indium tin oxide (ITO) electrode to be placed in a nitrogen condition; and cancer cell antibodies are decorated on the surface of the electrode to be placed in an incubator to be reserved. According to the number of cancer cells combined on the antibodies and linear changes of intensity of electrogenerated chemiluminescence, detection for the cancer cells is achieved. The electrogenerated chemiluminescence sensor and the fabrication method have the advantages of being simple in operation, high in reacting speed, good in effect, strong in renewability, strong in selectivity, and low in cost.

Description

technical field [0001] The invention relates to an electrochemiluminescence sensor for detecting cancer cells with high sensitivity and a preparation method thereof. Background technique [0002] Cancer is one of the important diseases that threaten human life, and liver cancer is a relatively common type of cancer. Therefore, how to detect and diagnose liver cancer early has become one of the research hotspots all over the world. At present, scientists at home and abroad use various methods to detect cancer cells, including ELISA, chemiluminescence, cyclic voltammetry, melt-out voltammetry, and chronoamperometry. However, the above methods have disadvantages such as relying on expensive analytical instruments, or taking a long time for analysis and detection, and cumbersome operations. Contents of the invention [0003] Aiming at the deficiencies of the prior art, the present invention provides an electrochemiluminescent sensor for detecting cancer cells with high sensit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/574
Inventor 韩晓军王磊马生华刘丹青
Owner HARBIN INST OF TECH
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