Cultural method of American ginseng adventitious root tissue

A cultivation method and technology of American ginseng, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem that the quality is easily affected by climate, cultivation conditions, diseases and insect pests, the supply is difficult to meet the market demand, and the cultivation and production cycle of American ginseng is long. and other problems, to achieve the effect of fast proliferation of adventitious roots, high yield and low cost

Inactive Publication Date: 2013-06-12
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cultivation and production cycle of American ginseng is long and covers a large area, and its quality is easily affected by climate, cultivation conditions, and pests and diseases. The s

Method used

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  • Cultural method of American ginseng adventitious root tissue
  • Cultural method of American ginseng adventitious root tissue
  • Cultural method of American ginseng adventitious root tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for culturing American ginseng adventitious root tissue, comprising the steps of:

[0029] (1) Induction of American ginseng callus:

[0030] Rinse the fresh roots of cultivated American ginseng with tap water, dry the surface in an oven at 45°C, then spray the surface with 75% ethanol in an ultra-clean bench, burn it with an alcohol lamp flame until it is dry for disinfection and sterilization, and then use sterilized Cut a 1cm incision on the surface of the root with the scalpel of the fungus, break it off from both sides, and then cut the internal tissue into 0.5-1cm 2 The small pieces were connected to the first solid medium prepared in advance, and cultured for 4 weeks under sterile, 23°C and dark conditions to form callus;

[0031] (2) Subculture of American ginseng callus:

[0032] Select a well-growing callus, inoculate it on the prepared second solid medium with a pre-sterilized scalpel and tweezers, and culture it under sterile, 23°C and dark condit...

Embodiment 2

[0044] A method for culturing American ginseng adventitious root tissue, comprising the steps of:

[0045] (1) Induction of American ginseng callus:

[0046] Take the sterilized fresh root of American ginseng and remove the tissue of the root bark, cut into 0.5 ~ 1cm 2 Inoculate small pieces of the first solid medium, and culture for 3.5 weeks under sterile, 24°C and dark conditions to form callus;

[0047] (2) Subculture of American ginseng callus:

[0048] The callus was inoculated on the second solid medium, and cultured for 3.5 weeks under sterile, 24°C and dark conditions;

[0049] (3) Induction of adventitious roots of American ginseng:

[0050] The subcultured callus obtained in step (2) was inoculated on the third solid medium, and cultured for 5 weeks under sterile, 24°C and light-proof conditions to cultivate adventitious roots of American ginseng;

[0051] (4) Subculture of adventitious roots of American ginseng:

[0052]The adventitious roots of American ginse...

Embodiment 3

[0060] A method for culturing American ginseng adventitious root tissue, comprising the steps of:

[0061] (1) Induction of American ginseng callus:

[0062] Take the sterilized fresh root of American ginseng and remove the tissue of the root bark, cut into 0.5 ~ 1cm 2 Inoculate small pieces of the first solid medium, and culture for 3 weeks under sterile, 25°C and dark conditions to form callus;

[0063] (2) Subculture of American ginseng callus:

[0064] The callus was inoculated on the second solid medium, and cultured under sterile, 25°C and dark conditions for 3 weeks;

[0065] (3) Induction of adventitious roots of American ginseng:

[0066] inoculating the subcultured callus obtained in step (2) on the third solid medium, culturing for 3 weeks under sterile, 25°C and light-proof conditions, and cultivating adventitious roots of American ginseng;

[0067] (4) Subculture of adventitious roots of American ginseng:

[0068] The adventitious roots of American ginseng ob...

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Abstract

The invention discloses a cultural method of American ginseng adventitious root tissue. The cultural method disclosed by the invention comprises the following steps: (1) inducing American ginseng callus; (2) secondarily culturing the American ginseng callus; (3) inducing the American ginseng adventitious root; (4) secondarily culturing the American ginseng adventitious root; and (5) culturing a reactor of the American ginseng adventitious root. According to the method disclosed by the invention, the growth velocity is much faster than the primary root. The root can be proliferated by 21.76 fold after culturing for a culture cycle; the productivity of the active secondary metabolite is high and stable; and meanwhile, the cultures are uniform and not affected by the natural environment such as natural disaster and the like. The cultural method of American ginseng adventitious root tissue disclosed by the invention has the characteristics of simple technical process, low cost, high inductivity, good repeatability and fast proliferation of the adventitious root.

Description

technical field [0001] The invention relates to a method for tissue culture of American ginseng, which belongs to the field of tissue culture of traditional Chinese medicine American ginseng. Background technique [0002] American ginseng (Panax quinquefolium L.), also known as American ginseng, is native to the eastern United States and Canada. Modern pharmacological studies have shown that American ginseng has the functions of regulating glucose metabolism, enhancing immunity, delaying aging, anti-tumor, lowering blood pressure, preventing and treating cardiovascular diseases, protecting liver and liver, enhancing learning ability and memory ability, and improving intelligence. Due to years of over-harvesting, the forest ecological environment on which American ginseng depends has been severely damaged, and the resources of wild American ginseng have been exhausted. Currently, cultivated American ginseng is mostly used for medicinal purposes. However, the cultivation and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高文远刘辉郭松波满淑丽王娟
Owner TIANJIN UNIV
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