Antimicrobial agent for tissue culture and manufacturing method thereof

Abstract

The invention discloses an antimicrobial agent for tissue culture and a manufacturing method thereof, relating to a chitin antimicrobial agent and belonging to the technical field of tissue culture. The invention is characterized in that the antimicrobial agent comprises the following components in percentage by weight (on the basis of 100 wt%): 40-60 wt% of chitin, 10-20 wt% of acrylamide, 10-20 wt% of chelator and 20-30 wt% of sorbitol. The invention has the following advantages: the chitin destroys the transcription from DNA (deoxyribonucleic acid) to RNA (ribonucleic acid), causing termination of bacterial reproduction; the negatively charged bacterial cell walls are adsorbed under the Coulomb action to inhibit breath, thereby causing death; and after the chitin is compounded with the synergists acrylamide and ethylenediamine tetraacetic acid (EDTA), the comprehensive antimicrobial effect is enhanced by nearly 30 times.

Description

technical field [0001] The invention relates to a chitin antibacterial agent, which belongs to the technical field of tissue culture, in particular to an antibacterial agent used in tissue culture and a preparation method thereof. Background technique [0002] Since the 1950s, plant tissue culture technology has developed rapidly and has been widely used in the rapid propagation of precious seedlings, the acquisition of non-toxic seedlings, the production of metabolites, and the cultivation and improvement of new plant varieties. plays a very important role. Plant tissue culture contamination is one of the biggest obstacles to tissue culture production, which directly affects production costs and the preservation of precious scientific research materials. the [0003] In the micro-ecological environment in the plant tissue culture container, pathogenic bacteria consume nutrients, produce toxic and harmful metabolites, and poison the culture. In addition, the culture itsel...

AI Technical Summary

Problems solved by technology

[0004] Inorganic fungicides are mainly silver-based fungicides, but the discoloration of silver ions has not been effectively controlled, resulting in great restrictions on the practical application of this type of fungicides

[0005] Organic fungicides are mostly chemical synthetic products, such as: chlorhexidine, quaternary ammonium salt, triclosan, etc., which are currently the most widely used fungicides. Such fungicides have defects such as poor safety, environmental pollution, and energy consumption.

[0006] The main varieties of natural fungicides are: chitosan, tea tree oil, sorbic acid, turmeric root alcohol, hinoki alcohol, Meng Zong bamboo extract, Coptis chinensis extract, etc. Due to the large effective dosage of this type of fungicide (high MIC), Expensive cost, poor water solubility, complex preparation process, unstable color and other problems have made it difficult to achieve industrial batch application so far, and most of them are still in the laboratory research stage. However, because it is derived from nature, it is especially safe for human application, which is in line with the human "return to nature" As a general trend, as a fungicide, it has always been an important category that the academic circles have devoted themselves to research and diligently pursued.