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Anti-tumor CTL (Cytotoxic T Lymphocyte) epitope peptide from MTA1 (Tumor Metastasis Associated Antigen 1) and application thereof

An epitope peptide, anti-tumor technology, applied in anti-tumor drugs, medical preparations containing active ingredients, peptides, etc.

Active Publication Date: 2014-03-26
郑州源创基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the HLA-class I restricted CTL epitope peptide targeting MTA1

Method used

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  • Anti-tumor CTL (Cytotoxic T Lymphocyte) epitope peptide from MTA1 (Tumor Metastasis Associated Antigen 1) and application thereof
  • Anti-tumor CTL (Cytotoxic T Lymphocyte) epitope peptide from MTA1 (Tumor Metastasis Associated Antigen 1) and application thereof
  • Anti-tumor CTL (Cytotoxic T Lymphocyte) epitope peptide from MTA1 (Tumor Metastasis Associated Antigen 1) and application thereof

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Experimental program
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Embodiment Construction

[0015] The anti-tumor CTL epitope peptide derived from MTA1 of the present invention includes a nonapeptide, and the amino acid sequence of the nonapeptide is

[0016] P22: Tyr-Leu-Ile-Arg-Arg-Ile-Glu-Glu-Leu

[0017] or P57: Ala-Leu-Ala-Asp-Lys-His-Ala-Thr-Leu

[0018] or P109: Phe-Leu-Ser-Arg-Gln-Leu-Glu-Ser-Leu

[0019] or P129: Thr-Leu-Leu-Asn-Glu-Thr-Glu-Ser-Leu

[0020] or P173: Tyr-Gln-Ala-Asp-Ile-Thr-Asp-Leu-Leu.

[0021] The present invention mainly adopts the method of combining theory and practice, according to the primary structure of the antigen, adopts immunoinformatics means, and uses SYFPEITHI, BIMAS, NetCTL 1.2 and IEDB databases to analyze the HLA-A*0201 restricted CTL epitope of the MTA1 protein antigen Predictive analysis was performed.

[0022] The above-mentioned epitope peptides were screened and synthesized using the standard Fmoc protocol. After HPLC purification, the purity was greater than 90%. Mass spectrometry analysis confirmed that the molecu...

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Abstract

The invention discloses an anti-tumor CTL (Cytotoxic T Lymphocyte) epitope peptide from MTA1 (Tumor Metastasis Associated Antigen 1). The epitope peptide comprises nonapeptide, the amino acid sequence of which is P109:Phe-Leu-Ser-Arg-Gln-Leu-Glu-Ser-Leu. The invention further comprises the application of the peptide for preparing polypeptide vaccines for treating tumor. The invention has the advantages that the epitope peptide of tumor metastasis associated antigen is initially identified by a method combining theory with experiment. The nonapeptide identified is not reported by documents, so that a theoretical base for researching antigen MTA1 based polypeptide vaccines for treating tumor is provided and a foundation for constructing subsequent multivalent antigen peptide vaccines, long peptide vaccines, multi-epitope vaccines and the like is laid.

Description

[0001] The present invention is a patent application with patent application number: 201110138635.4, application date: May 26, 2011, and a divisional application with the invention name "Anti-tumor CTL epitope peptide derived from MTA1 and its application". technical field [0002] The present invention relates to peptides, especially anti-tumor CTL epitope peptides derived from MTA1, and also relates to the application of the peptides in preparing tumor therapeutic polypeptide vaccines. Background technique [0003] Metastasis is a complex process involving multiple causes and their products, including detachment of tumor cells from the primary tumor, invasion of lymphatic vessels, blood vessels, and surrounding tissues, induction of angiogenesis, evasion of host cell antitumor responses, and growth at metastatic sites. Therefore, understanding the genes and gene products involved in tumor metastasis is a hot research topic at home and abroad. [0004] Metastasis-associated...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K39/00A61P35/00
Inventor 祁元明吴亚红高艳锋陈飞孙萌韩艳林时冉冉陈艳平
Owner 郑州源创基因科技有限公司