Culture medium for producing oxytetracycline through streptomyces rimosus fermentation and fermentation method

A technology of Streptomyces fissures and fermentation medium, which is applied in the medium and fermentation field of Streptomyces fissures to produce oxytetracycline, which can solve the problems of affecting product quality, high cost and pressure of procurement, and complicated process control. To optimize the formula of the medium, to facilitate the fermentation culture, and to improve the effect of the fermentation unit

Active Publication Date: 2013-06-12
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peanut cake powder and cottonseed cake powder are used as the nitrogen source of the fermentation medium. Due to their poor quality and high content of impurities, which affect the metabolites of the strains, the color of the fermentation broth is darkened, and the fermentation unit is low. The fermentation unit is generally 20,000 to 23,000. Ultimately affect product quality; if the proportion of animal protein compatibility is high, a large amount of foam will be generated during the fermentation process. If it is not strictly controlled, the fermentation liquid will escape and cause bacterial contamination
A

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Primary seed medium preparation: seed tank volume 1m 3 , the effective volume is 0.8m 3 . Configure two first-level seed media respectively.

[0065] (No. 1) primary seed medium preparation process: 2.4L corn steep liquor, 4kg low-temperature soybean cake powder, 4.8kg ammonium sulfate, 12kg corn starch, 1.6kg calcium carbonate, 0.72kg sodium chloride, 0.24kg potassium dihydrogen phosphate, Cobalt chloride 3.2g, yeast powder 4kg, maltodextrin 12kg, corn oil 12kg. The volume of primary seed liquid is 0.8m 3 .

[0066] (No. 2) Primary seed medium preparation process: 1.2L corn steep liquor, 2kg low-temperature soybean cake powder, 2.4kg ammonium sulfate, 6kg corn starch, 0.8kg calcium carbonate, 0.36kg sodium chloride, 0.12kg potassium dihydrogen phosphate, Cobalt chloride 1.6g, yeast powder 2kg, maltodextrin 6kg, corn oil 6kg. The volume of primary seed liquid is 0.4m 3 .

[0067] Secondary seed medium preparation: seed tank volume 10m 3 , the effective volume...

Embodiment 2

[0082] Primary seed medium preparation: seed tank volume 1m 3 , the effective volume is 0.8m 3 . Configure two first-level seed media respectively.

[0083] (No. 1) Primary seed medium preparation process: corn steep liquor 2.8L, low-temperature soybean cake powder 6kg, ammonium sulfate 5.6kg, corn starch 14kg, calcium carbonate 2kg, sodium chloride 0.76kg, potassium dihydrogen phosphate 0.28kg, chlorine Cobalt 4.4g, yeast powder 6kg, maltodextrin 14kg, corn oil 14kg. The volume of primary seed liquid is 0.8m 3 .

[0084] (No. 2) Primary seed medium preparation process: 1.4L corn steep liquor, 3kg low-temperature soybean cake powder, 2.8kg ammonium sulfate, 7kg corn starch, 1kg calcium carbonate, 0.38kg sodium chloride, 0.14kg potassium dihydrogen phosphate, chlorine Cobalt 2.2g, yeast powder 3kg, maltodextrin 7kg, corn oil 7kg. The volume of primary seed liquid is 0.4m 3 .

[0085] Secondary seed medium preparation: seed tank volume 10m 3 , the effective volume is ...

Embodiment 3

[0099] Primary seed medium preparation: seed tank volume 1m 3 , the effective volume is 0.8m 3 . Configure two first-level seed media respectively.

[0100] (No. 1) Primary seed medium preparation process: 3.2L corn steep liquor, 8kg low-temperature soybean cake powder, 6.4kg ammonium sulfate, 16kg corn starch, 2.4kg calcium carbonate, 0.8kg sodium chloride, 0.32kg potassium dihydrogen phosphate, Cobalt chloride 5.6g, yeast powder 8kg, maltodextrin 16kg, corn oil 16kg. The volume of primary seed liquid is 0.8m 3 .

[0101] (No. 2) Primary seed medium preparation process: 1.6L corn steep liquor, 4kg low-temperature soybean cake powder, 3.2kg ammonium sulfate, 8kg corn starch, 1.2kg calcium carbonate, 0.4kg sodium chloride, 0.26kg potassium dihydrogen phosphate, Cobalt chloride 2.8g, yeast powder 4kg, maltodextrin 8kg, corn oil 8kg. The volume of primary seed liquid is 0.4m 3 .

[0102] Secondary seed medium preparation: seed tank volume 10m 3 , the effective volume i...

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Abstract

The invention relates to a culture medium for producing oxytetracycline through streptomyces rimosus fermentation and a fermentation method. According to the invention, by replacing conventional soybean cake powder with low-temperature soybean cake powder, stopping animal protein substances and optimizing the formula of the culture medium, the problem in the cost of the raw and accessory materials is solved, the environmental influence on the source of the raw and accessory materials is minimized, sufficient supply is guaranteed, and stable and efficient production of oxytetracycline is realized. Meanwhile, by adopting the culture medium and method provided by the invention to produce oxytetracycline, the fermentation unit of oxytetracycline can be increased to over 34000u/ml, which is increased by over 6% in comparison with the domestic conventional technologies; and compared with the prior art, the fermentation period is shortened by over 5%, the oxytetracycline output is increased, the production efficiency is improved, and the aim of saving energy and reducing consumption is achieved.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a fermentation method for producing oxytetracycline by fermentation of Streptomyces crackis. Background technique [0002] Oxytetracycline is a broad-spectrum antibiotic, which has inhibitory effects on Gram-positive bacteria and Gram-negative bacteria, and also has certain inhibitory effects on Chlamydia, Rickettsia, Mycoplasma, Spirochetes, etc. Oxytetracycline is mainly used to prevent and treat Escherichia coli, Salmonella infection, Pasteurella, Brucella infection, swine panting disease, and chicken chronic respiratory disease; it is also commonly used to treat dog rickettsiasis and cat infectious Anemia, prevention of chlamydial disease and leptospirosis in dogs; locally used for endometritis and tissue necrosis caused by Necroptosis bacillus; used as a feed lick additive to promote growth and improve feed conversion rate. [0003] Acc...

Claims

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Application Information

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IPC IPC(8): C12P29/00C12R1/59
Inventor 王义任勇王文超
Owner 宁夏泰瑞制药股份有限公司
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