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SNP marker based typing method for spartina alterniflora population

A technology of Spartina alterniflora and marker method, which is applied in the field of molecular marker screening of Spartina alterniflora, achieving the effects of high success rate, accurate results and stable genetics

Inactive Publication Date: 2013-06-12
NANJING UNIV
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Problems solved by technology

But at present, most of the researches are mainly focused on ecological processes such as competitive exclusion, niche replacement, biological characteristics, allelopathy, predation, parasitism, etc., lacking the traceability of its population, rapid adaptation and evolution model, rapid invasion and expansion dynamics, etc. Understanding of Intrusion Mechanisms

Method used

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  • SNP marker based typing method for spartina alterniflora population
  • SNP marker based typing method for spartina alterniflora population
  • SNP marker based typing method for spartina alterniflora population

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with specific samples, which are explanations of the present invention rather than limitations.

[0024] 1. Population survey and sampling

[0025] Select Chongming Island in Shanghai and the coast of Tanggu Haihe River in Tianjin as sampling points, select leaves that grow well and have no pests, put them in a pre-prepared ziplock bag with about half a bag of silica gel immediately after picking, and mix well to make the leaves and silica gel fully Mix for quick dehydration. After taking it back to the laboratory, replace the discolored silica gel according to the actual situation, and after drying, it will be used for DNA extraction. About 30 plants are randomly sampled at each sampling point, and the distance between individuals is not less than 50 meters, so as to avoid repeated collection of the same clone as much as possible, and the number, habitat, geographical parameters (latitude a...

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Abstract

The invention relates to an SNP (single nucleotide polymorphism) marker based typing method for a spartina alterniflora population. According to the method, a target gene can be screened based on high-throughput transcriptome sequencing, and then by means of a PCR reaction, PCR product sequencing, multiple SNP loci can be obtained. Then, all valid SNP loci compose a haplotype to be used for molecular markers, thus achieving genetic typing. According to the method disclosed in the invention, abundant polymorphic information can be acquired in one experiment. The method provided in the invention has the advantages of genetic stability of SNP markers and simple operation, thus being suitable for species origin tracing.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a screening technology of molecular markers of Spartina alterniflora based on SNP markers. Background technique [0002] Spartina alterniflora is a perennial herb native to the Atlantic coast of North America. It has been introduced in many countries because of its remarkable effect of promoting silting and land-building and eliminating waves and embankments. In 1979, Nanjing University introduced three different ecological types of Spartina alterniflora from North Carolina, Georgia and Florida in the southeastern United States to Luoyuan Bay, Fujian, and then expanded their distribution to the vast intertidal zone of my country's coastal intertidal zone south of Dandong, Liaoning. area, and achieved certain ecological and economic benefits. However, as an alien species, its strong reproductive ability and tall and dense population characteristics have had a relatively large negative ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCY02A90/40
Inventor 陈建群丁静王强许颖吴娟子
Owner NANJING UNIV
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