Identification method for ginkgo seedling male and female plants

Abstract

The invention provides an identification method for ginkgo seedling male and female plants, and the method is mainly directed at identification of the seedling stages of ginkgo seedling male and female plants in urban garden greening. By preparing a phosphate buffer solution, utilizing the chromogenic reaction of a bromothymol blue solution, and a proper treatment during a seedling stage, various conditions needed by early identification of ginkgo seedling male and female plants can be guaranteed. Comprising the steps of grinding, extraction, centrifugation and the like on the ginkgo seedling male and female plants, the provided method can be applied in large areas during production practice of urban garden greening, and can solve the identification problem of ginkgo seedling male and female plants. Being effective, the method has the advantages of simple operation, high accuracy, reduction of identification time, and cost saving.

Description

technical field [0001] The invention relates to a method for identifying male and female plants of plants, in particular to an early identification method for male and female ginkgo plants. Background technique [0002] Most plants in nature are monoecious, but a few tree species such as ginkgo are dioecious; the male ginkgo only blooms but not bears fruit, and the female ginkgo will bear fruit; even if the branches of the female ginkgo are grafted to the male ginkgo tree, there is only one The branches bear fruit. Generally, it is difficult to distinguish male and female ginkgo trees from the appearance. There are only slight differences in tree shape between the two. The canopy of the female ginkgo expands to both sides, while the canopy of the male ginkgo grows desperately upwards. Only professionals in the industry can distinguish between male and female plants. Due to the lack of fruit, male ginkgo trees are more felled. Therefore, in nature, there are more female gin...

AI Technical Summary

Problems solved by technology

Isozyme spectrum and chromosome karyotype identification methods have high accuracy, but the difference in enzyme bands is not necessarily caused by male and female sex, but may be related to the variation of other traits, such as leaf traits, etc. These variations are common in male and female Ginkgo plants , and because in the early identification, there are fewer enzymes to choose from, and there are not many colored enzyme bands, sometimes it is impossible to distinguish between male and female plants

Moreover, the operation process is complicated, requiring CTAB, PVP, Tris-HCl, phenol, chloroform, TE, ethidium bromide and other drugs. The drugs are expensive and highly toxic, and primers and PCR instruments are required. It is difficult for ordinary laboratories to operate with knowledgeable people

Therefore, the above methods are not suitable for promotion in urban landscaping projects.