Nano-gold-based method for visually and rapidly detecting antibiotics in milk

A nano-gold and antibiotic technology, applied in the field of food analysis, can solve the problems of long time required, cumbersome operation process, expensive chromatography equipment, etc., and achieve the effect of good selectivity, high sensitivity and simple operation

Inactive Publication Date: 2013-06-12
UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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  • Abstract
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Problems solved by technology

[0003] At present, the amount of antibiotics used in milk is strictly limited. Finding a method that can detect multiple antibiotics in milk at the same time is an urgent need to ensure food safety.
At present, the methods for detecting antibiotics include traditional methods of inhibiting microbial growth, liquid chromatography, gas chromatography, capillary electrophoresis, and some new methods developed in recent years...

Method used

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  • Nano-gold-based method for visually and rapidly detecting antibiotics in milk
  • Nano-gold-based method for visually and rapidly detecting antibiotics in milk
  • Nano-gold-based method for visually and rapidly detecting antibiotics in milk

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Embodiment Construction

[0019] 1. Use disodium hydrogen phosphate and sodium dihydrogen phosphate to configure buffer solution, and use secondary water to prepare solutions such as catechol violet, gold chloride, antibiotics, etc.; add gold chloride solution, dodecyl three Methyl ammonium bromide (DTAB), catechol violet (PCV), after shaking up, observe the change of solution color, and measure its ultraviolet-visible spectrum figure;

[0020] 2. Add gold chloride solution, dedecyltrimethylammonium bromide (DTAB), different concentrations of antibiotic aqueous solution, or milk sample solution with known concentration of antibiotics, catechol purple , after shaking well, observe the change of solution color, and measure its ultraviolet-visible spectrum figure;

[0021] 3. The solvent used is 0.01M, pH=7.0 phosphate buffer solution, the concentrations of catechol violet, chloroauric acid and DTAB are respectively 8×10 -5 M, 1.0×10 -4 M and 1.0×10 -6 M.

[0022] 4. When no antibiotics were added, th...

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Abstract

The invention provides a nano-gold-based method for visually and rapidly detecting antibiotics in milk. The method disclosed by the invention is mainly characterized in that pyrocatechol violet is used for reducing chloroauric acid to prepare the nano-gold. In a nano-gold synthesizing process, the addition of the antibiotics (including kanamycin mono-sulfate, neomycin sulfate, streptomycin sulfate and bleomycin sulfate) influences the synthesis of the nano-gold so that the color is changed; and the antibiotics can be quantitatively detected through naked eyes and an ultraviolet visible absorption spectrometer. According to the method, the operation is simple and rapid, the sensitivity is high, the selectivity is good, and the method can be applied to the detection of the antibiotics in the milk.

Description

technical field [0001] The invention relates to a food safety analysis method, which specifically refers to the combined use of naked eyes and ultraviolet-visible spectrometers for detecting the residual antibiotic content in milk. The method belongs to the field of food analysis. Background technique [0002] Antibiotics are one of the most commonly used drugs in clinical practice. They have miraculous effects on the prevention and treatment of infectious diseases in animals and humans. However, the excessive use of antibiotics will bring serious harm to human life, such as allergic reactions caused by penicillin and bacterial resistance. The misuse of antibiotics has become a global public health problem. In order to ensure the safety of consumers, some international organizations and food and drug regulatory authorities have clearly regulated the dosage of antibiotics, and have regulated the maximum residue of some antibiotics. For example, in milk, the amount of kanamy...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31G01N21/33
Inventor 赵红张晓芳张阳何裕建李向军袁倬斌
Owner UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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