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Paenibacillus polymyxa microbial fertilizer produced through resource production of surplus sludge

A technology of polymyxa-like spores and microbial fertilizers, applied in the direction of organic fertilizers, can solve problems such as soil deterioration, polluted water bodies and the environment, and excessive agricultural waste

Active Publication Date: 2015-05-13
鹤岛控股集团有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent (application number: 02133713.6) discloses that Paenibacillus polymyxa can be used as a component in microbial fertilizer inoculum to decompose straw, which effectively solves the problem of excessive agricultural waste in agricultural production, which pollutes water bodies and the environment and is difficult to handle. Excessive use of chemical fertilizers and pesticides, the contradiction of soil deterioration
There is no literature report on the method of producing Paenibacillus polymyxa microbial fertilizer with surplus sludge as the main medium raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: the surplus sludge that domestic sewage treatment produces produces Paenibacillus polymyxa microbial fertilizer

[0016] 1. Pretreatment of excess sludge: Select the excess activated sludge of domestic sewage treatment plants whose heavy metal content does not exceed the agricultural standard as raw material, mix the undewatered sludge and dewatered sludge in the secondary settling tank, and adjust to the sludge concentration 50g / L; transport the uniformly stirred sludge suspension to a microwave reactor, heat it with microwaves to 100°C, and react for 15 minutes. After stopping the heating, cool naturally to room temperature to obtain the remaining sludge medium.

[0017] 2. Preparation of liquid seeds: inoculate Paenibacillus polymyxa preserved on a slant into a conical flask equipped with potato liquid medium, and vibrate at 33° C. at 150 rpm for 24 hours to obtain liquid seeds.

[0018] 3. Liquid fermentation: Inoculate liquid seeds with a volume ratio...

Embodiment 2

[0020] Embodiment 2: the surplus sludge that brewery wastewater treatment produces produces Paenibacillus polymyxa microbial fertilizer

[0021] 1. Excess sludge pretreatment: Select the excess activated sludge produced by brewery wastewater treatment whose heavy metal content does not exceed the agricultural standard as raw material, mix the excess sludge with brewery wastewater, and adjust the sludge concentration to 35g / L; stir The uniform sludge suspension is transported to a microwave reactor, heated by microwave to 100°C, and reacted for 10 minutes. After stopping the heating, it is naturally cooled to room temperature to obtain the remaining sludge medium.

[0022] 2. Preparation of liquid seeds: Inoculate Paenibacillus polymyxa preserved on a slant into a conical flask equipped with potato liquid medium, and vibrate at 32° C. at 150 rpm for 30 hours to obtain liquid seeds.

[0023] 3. Liquid fermentation: Inoculate liquid seeds with a volume ratio of 5% in a fermentati...

Embodiment 3

[0025] Embodiment 3: Paenibacillus polymyxa microbial fertilizer is applied to rapeseed production

[0026] Using the vegetable greenhouse plot experiment, set up 8 experimental plots with exactly the same conditions, each plot 60m 2, 2 plots are 1 group of parallel experiments, divided into 4 groups, planting rapeseed, in the rapeseed seedling stage, the 2 plots of the 1st group are not fertilized as a control; Paenibacillus polymyxa microbial fertilizer was topdressed once after germination and 3 weeks later; the 2 plots of the 3rd group were applied urea once after the complete germination of rapeseed and 3 weeks later according to the fertilization rate of 15 kg / mu; the 4th group 2 According to the fertilization rate of 15 kg / mu, the urea was applied once after the rape fully germinated and after 3 weeks, and the microbial fertilizer was applied once according to the fertilization rate of 200 kg / mu one day after each application of urea. Irrigation and management methods....

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Abstract

The invention relates to a paenibacillus polymyxa microbial fertilizer produced through the resource production of surplus sludge, which belongs to the technical field of microbial fertilizers and sludge resource. The method is implemented through mainly taking surplus sludge produced in the process of sewage treatment as a main culture medium material, and the paenibacillus polymyxa microbial fertilizer produced by using a liquid fermentation method. The production process comprises the following steps: (1) preparing a fermentation culture medium through carrying out microwave heating pretreatment on surplus sludge; (2) preparing paenibacillus polymyxa liquid seeds; (3) inoculating the liquid seeds in a fermentation tank filled with the surplus sludge fermentation culture medium, and feeding sterile air into the fermentation tank to culture, thereby obtaining a liquid fermentation product; and (4) mixing fermented liquid with a high-quality organic fertilizer carrier so as to obtain a microbial fertilizer. The paenibacillus polymyxa microbial fertilizer provided by using the process has the characteristics of low production cost and environmental friendliness, and products can significantly improve the quality of soil environment, increase the yield and quality of crops, and also can prevent and control various plant diseases. Meanwhile, an effective resource utilization method is found for large amounts of surplus sludge produced in the process of sewage treatment, thereby reducing pollutant emissions.

Description

Technical field: [0001] The invention belongs to the technical field of microbial fertilizer and sludge resource utilization. Background technique: [0002] In the sewage treatment process, the activated sludge method is one of the most widely used technologies. This method plays a very important role in water treatment because of its advantages of high efficiency, low cost and less land occupation. However, this method During the process of sewage treatment, a large amount of residual sludge will be produced, usually accounting for 0.5% to 1.0% of the total sewage. In an ordinary secondary sewage treatment plant, the investment required for sludge treatment accounts for about 30% to 40% of the total investment, and the operating cost accounts for about 20% to 50% of the total operating cost. Sludge digestion, dehydration, and then landfill is a commonly used disposal method in domestic large-scale sewage treatment plants. Landfill disposal is more economical, but this dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08
Inventor 王立国白志辉焦海华张茉莉张瑛瑛
Owner 鹤岛控股集团有限公司