Acute B-lymphocyte leukemia initiated cell phenetic classification kit and application thereof

A technology of B lymphocytes and classification reagents, which is applied in the field of kits for detecting acute B lymphocytic leukemia to start cell phenotype classification, and achieves the effect of rapid typing

Active Publication Date: 2013-06-26
PEOPLES HOSPITAL PEKING UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, in order to identify and classify the LICs of B-ALL patients from normal B progenitor cells, and find the specific surface markers of LICs and their processing steps and methods, there are no reports at home and abroad.

Method used

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  • Acute B-lymphocyte leukemia initiated cell phenetic classification kit and application thereof
  • Acute B-lymphocyte leukemia initiated cell phenetic classification kit and application thereof
  • Acute B-lymphocyte leukemia initiated cell phenetic classification kit and application thereof

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Experimental program
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Embodiment Construction

[0026] The present invention provides a kit for initiating cell phenotype classification for acute B lymphocytic leukemia, which includes the following fluorescently labeled monoclonal antibody reagents: CD58, CD10, CD34, CD19, CD45 and CD38, respectively corresponding to the following fluorescently labeled FITC, PE , PerCP, APC-Cy7, Pacific Blue, and APC. See Table 1 for information on fluorescent labels and components of each monoclonal antibody.

[0027] Table 1 Flow Cytometry Monoclonal Antibody Information

[0028] name fluorescent label Element clone catalog number company CD58 FITC Mouse IgG2a AICD58 IM1218 BECKMAN COULTER CD10 PE Mouse IgG1 HI10a 340921 BD CD34 PerCP Mouse IgG1 8G12 340430 BD CD19 APC-Cy7 Mouse IgG1 SJ25C1 348794 BD CD45 Pacific Blue Mouse IgG1 HI30 304022 Biolegend CD38 APCs Mouse IgG1 HB7 345807 BD

[0029] The kit is also pr...

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Abstract

The invention discloses an acute B-lymphocyte leukemia initiated cell phenetic classification kit. The kit is used in cooperation with a sever-color flow cytometer. The structure of the kit is configured with the following fluorescently-labeled monoclonal antibody: CD58FITC, CD10PE, CD34PerCP, CD38APC, CD19APC-Cy7 and CD45PacificBlue. The kit disclosed by the invention can be used for fast and accurately detecting the immunophenotype of the leukemia initiated cell of a B-ALL patient in first visit; and the CD58 expression proportion on the leukemia initiated cell tested by the kit provided by the invention can be used as one of the prognostic indicators of the B-ALL patient, and the indicator has important guiding significance in the prognosis of the B-ALL patient and the decision of a clinic treatment scheme.

Description

technical field [0001] The invention belongs to the structural design of a special kit in the process of cell phenotype confirmation, and in particular relates to a kit for detecting acute B lymphocytic leukemia to initiate cell phenotype classification and its application. Background technique [0002] Acute lymphoblastic leukemia (ALL) is one of the most common hematological malignancies in the world. It is a major disease that seriously endangers the health of the people, especially children and young adults. It is of great significance to improve its cure rate. The optimization of treatment regimens in the past half century has significantly improved the induction remission rate and long-term survival rate of children with ALL, and the 5-year disease-free survival (DFS) has reached 70-80%. ALL's 5-year DFS hovers around 30%. Relapse is the main cause of treatment failure in ALL patients and an important problem affecting the long-term survival of ALL patients. Huge med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 黄晓军孔圆刘艳荣江倩江浩王亚哲主鸿鹄郝乐常艳刘代红许兰平刘开彦
Owner PEOPLES HOSPITAL PEKING UNIV
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