Reagent device for detecting anti-SSA antibody and method thereof

Abstract

The invention discloses a reagent device for detecting an anti-SSA antibody and a method thereof; the reagent device is long strip-shaped, comprises a substrate with eight hole sites and a handle disposed at one end of the substrate; the eight hole sites comprise a sample hole, an adjuvant hole, an enzyme conjugate hole, a substrate hole, a stopping solution hole, a diluent hole, a reaction hole, and a dilution hole, which are arranged in sequence from the end near the handle. The reagent device of the invention realizes anti-SSA antibody detection with the device based on a principle of enzyme-linked immunoassay, is an independent, single-sample analytic detection method, and can be used cooperatively with corresponding specific analytical instruments; during detection, a detection reagent or a sample is injected by using a full automatic precision liquid filling device, which provides advantages of operation automation, accurate filling amount, high accuracy and precision of detection results; and the reagent device has wide application prospects.

Description

technical field [0001] The application of the present invention relates to a reagent device and a method for detecting anti-SSA antibodies, belonging to the technical field of clinical immunology detection. Background technique [0002] SSA is a ribonucleoprotein, RNA protein particle widely distributed in all tissues, which consists of two proteins (60kDa and 52kDa). Anti-SSA antibodies were first found in the serum of patients with Sjogren's syndrome. [0003] Anti-SSA antibodies mainly appear in subtypes associated with systemic lupus erythematosus, such as subacute cutaneous lupus erythematosus, neonatal lupus erythematosus, etc.; human IgG containing anti-SSA antibodies can induce conduction block in the heart of neonatal rabbits, and can also induce heart rate Abnormalities such as slowing down and heart block. [0004] Common methods for clinical detection of anti-SSA antibodies include immunoprecipitation, indirect immunofluorescence, and enzyme-linked immunosorben...

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Problems solved by technology

[0009] (1) Non-specific recognition cannot be distinguished according to the size of the molecular weight when analyzing the results;

[0010] (2) The operation is relatively complicated and requires expensive fluorescence microscopes, which are difficult to promote in many primary hospitals and are not suitable for laboratories with a large number of specimens;

[0011] (3) The background in the fluorescence measurement is relatively high, and it is difficult to use the fluorescence immunoassay technique for quantitative determination;

[0012] (4) Experienced professionals are required to determine the results, and the objectivity of the analysis results is insufficient;

[0013] (5) Only qualitative detection can be carried out, and quantitative measurement cannot be carried out

[0016] (1) Use 12×8 type, 6×8 type, 8×12 type or full-plate type 96-well special microwell plate as antigen coating equipment and reaction container, which can only be divided into 12 batches and 6 batches when used , 8 batches or the whole board can be used at one time, and independent and single-person testing cannot be carried out;

[0017] (2) There are many kinds of reagents used in quantitative determination, and each detection reagent must be contained in a reagent bottle, and each time a reagent is used, the suction nozzle needs to be replaced to fill the microwells of the microwell plate respectively , ...

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