Method for improving integrity of cytoskeletons after freeze thawing of preserved boar sperm

Abstract

The invention discloses a method for improving the integrity of cytoskeletons after freeze thawing of preserved boar sperm. The method comprises the following steps of: collecting the boar sperm from an adult, healthy and carnal exuberant boar; extracting low density lipoprotein (LDL) from pigeon eggs; and freezing the boar sperm by using the LDL as a freezing protective agent. By adopting the method for improving the integrity of the cytoskeletons after freeze thawing of the preserved boar sperm, 9 percent LDL serving as the freezing protective agent is added, so that the integrity of the cytoskeletons after freeze thawing of the boar sperm is improved, the expression quantity of actin in the cytoskeletons of the boar sperm can be obviously improved, meanwhile, the expression quantity of tubulin is closer to that of fresh sperm, the survival rate, vitality and fertilization rate of the freeze-thawed boar sperm are effectively improved, the requirements of real use are furthest met, and the method has relatively strong popularization and application values.

Description

technical field [0001] The invention belongs to the technical field of biology, and in particular relates to a method for improving the integrity of the cytoskeleton after freezing and thawing preserved pig sperm. Background technique [0002] After freezing and thawing pig spermatozoa, a large number of spermatozoa died or their motility decreased due to a series of freezing injuries, and when egg yolk was used as a cryoprotectant, the expression of actin and tubulin in pig sperm cytoskeleton was not sufficient Ideal, can not meet the actual use requirements. [0003] One of the main reasons for the low fertilization rate of frozen-thawed sperm is damage to the cytoskeleton. Studies have shown that low-density lipoprotein LDL can improve the freezing efficiency of frozen-thawed sperm, but there are few reports on its effect on the integrity of the frozen-thawed sperm cytoskeleton. Contents of the invention [0004] The invention provides a method for improving the integ...

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for improving the integrity of the cytoskeleton after freezing and thawing pig spermatozoa, aiming to solve the problem of using egg yolk as cryoprotection due to a series of freezing damages resulting in a large number of sperm deaths or decreased vitality after freezing and thawing pig spermatozoa. The expression level of actin and the amount of tubulin in the pig sperm cytoskeleton are not ideal, and cannot meet the requirements of actual use.

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